Anti-alpha+beta SNAP antibody [6B7-3] (ab69742)

Overview

  • Product name
    Anti-alpha+beta SNAP antibody [6B7-3]
    See all alpha+beta SNAP primary antibodies
  • Description
    Mouse monoclonal [6B7-3] to alpha+beta SNAP
  • Host species
    Mouse
  • Specificity
    This antibody detects a doublet of 35 and 36 kDa proteins, corresponding to the apparent molecular mass of alpha + beta SNAP.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human
  • Immunogen

    Recombinant full length bovine alpha SNAP protein expressed in E coli

  • Positive control
    • Rat brain tissue extract and mouse brain tissue extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab69742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.5 µg/ml. Predicted molecular weight: 38 kDa.
IP Use at an assay dependent dilution.

Target

  • Relevance
    Alpha, beta and gamma SNAPs, also known as soluble NSF attachment proteins, have apparent molecular weights on SDS PAGE of 35, 36 and 39 kDa, respectively. SNAP proteins mediate the association of NSF to membranes during vesicle fusion reaction, and play a prominent role in synaptic vesicle fusion. In addition, beta SNAP interacts with synaptotagmin and may be involved in calcium regulated exocytosis. SNAP was identified as being required for vesicle fusion in intra Golgi transport reaction in vitro. Alpha SNAP mediates the association of NSF to membranes during vesicle fusion reaction, and plays a prominent role and in synaptic vesicle fusion. Together with synaptotagmin and NSF, alpha SNAP modulates the interaction of SNAP25, VAMP, and syntaxin in, an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.
  • Cellular localization
    Cytoplasmic peripheral membrane protein.
  • Database links
  • Alternative names
    • Alpha soluble NSF attachment protein antibody
    • Beta soluble NSF attachment protein antibody
    • N ethylmaleimide sensitive factor attachment protein alpha antibody
    • N ethylmaleimide sensitive factor attachment protein beta antibody
    • NAPA antibody
    • NAPB antibody
    • SNAP Alpha antibody
    • SNAP Beta antibody
    • SNAPA antibody
    • SNAPB antibody
    • SNARE antibody
    • Soluble NSF attachment protein antibody
    see all

References

ab69742 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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