Purification notesWhole antiserum was fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceAlpha, beta and gamma SNAPs, also known as soluble NSF attachment proteins, have apparent molecular weights on SDS PAGE of 35, 36 and 39 kDa, respectively. SNAP proteins mediate the association of NSF to membranes during vesicle fusion reaction, and play a prominent role in synaptic vesicle fusion. In addition, beta SNAP interacts with synaptotagmin and may be involved in calcium regulated exocytosis. SNAP was identified as being required for vesicle fusion in intra Golgi transport reaction in vitro. Alpha SNAP mediates the association of NSF to membranes during vesicle fusion reaction, and plays a prominent role and in synaptic vesicle fusion. Together with synaptotagmin and NSF, alpha SNAP modulates the interaction of SNAP25, VAMP, and syntaxin in, an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.
N ethylmaleimide sensitive factor attachment protein alpha antibody
N ethylmaleimide sensitive factor attachment protein beta antibody
SNAP Alpha antibody
SNAP Beta antibody
Soluble NSF attachment protein antibody
Anti-alpha+beta SNAP antibody images
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-alpha+beta SNAP antibody (ab50483)Image from Yu Y et al, Mol Cell Proteomics. 2009 Jul;8(7):1490-500. Epub 2009 Mar 11, Fig 6. DOI 10.1074/mcp.M800273-MCP200
ab50483 at a 1/500 dilution staining alpha+beta SNAP in murine brain tissue by Immunohistochemistry (PFA perfusion fixed frozen sections). Mice were euthanized, and the brains were exposed and removed from the body. Brain tissue was fixed in 4% polyformaldehyde. Fixed brain tissues were cut into transverse sections 5 µm thick. The sections were collected on poly-l-lysine-coated slides and air-dried. After blocking, sections were incubated with ab50483 in PBS for 2 hours at 37 °C in a humidified chamber. After washing, slides were incubated with the secondary antibody, a rabbit anti-goat IgG conjugated with horseradish peroxidase for 1 hour at 37 °C in a humidity box.
References for Anti-alpha+beta SNAP antibody (ab50483)
This product has been referenced in:
Yu Y et al. Evaluation of blastomere biopsy using mouse model indicates the potential high-risk of neurodegenerative disorders in the offspring. Mol Cell Proteomics : (2009).
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