Overview

  • Product name
    Anti-alpha+beta SNAP antibody
    See all alpha+beta SNAP primary antibodies
  • Description
    Rabbit polyclonal to alpha+beta SNAP
  • Tested applications
    Suitable for: IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus laevis, Pufferfish, Zebrafish, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    KAIAHYEQSADYYKGEES

    conjugated to KLH, corresponding to internal sequence amino acids 140-157 of Mouse alpha+beta SNAP

  • Positive control
    • Cytosolic fraction S1 of rat and mouse brain

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Whole antiserum was fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
WB
  • Application notes
    IHC-FoFr: 1/75 (PMID 19279043).
    WB: 1/1000 - 1/2000. Predicted molecular weight: 38 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Alpha, beta and gamma SNAPs, also known as soluble NSF attachment proteins, have apparent molecular weights on SDS PAGE of 35, 36 and 39 kDa, respectively. SNAP proteins mediate the association of NSF to membranes during vesicle fusion reaction, and play a prominent role in synaptic vesicle fusion. In addition, beta SNAP interacts with synaptotagmin and may be involved in calcium regulated exocytosis. SNAP was identified as being required for vesicle fusion in intra Golgi transport reaction in vitro. Alpha SNAP mediates the association of NSF to membranes during vesicle fusion reaction, and plays a prominent role and in synaptic vesicle fusion. Together with synaptotagmin and NSF, alpha SNAP modulates the interaction of SNAP25, VAMP, and syntaxin in, an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.
    • Cellular localization
      Cytoplasmic peripheral membrane protein.
    • Database links
    • Alternative names
      • Alpha soluble NSF attachment protein antibody
      • Beta soluble NSF attachment protein antibody
      • N ethylmaleimide sensitive factor attachment protein alpha antibody
      • N ethylmaleimide sensitive factor attachment protein beta antibody
      • NAPA antibody
      • NAPB antibody
      • SNAP Alpha antibody
      • SNAP Beta antibody
      • SNAPA antibody
      • SNAPB antibody
      • SNARE antibody
      • Soluble NSF attachment protein antibody
      see all

    Anti-alpha+beta SNAP antibody images

    • ab50483 at a 1/500 dilution staining alpha+beta SNAP in murine brain tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).
      Mice were euthanized, and the brains were exposed and removed from the body. Brain tissue was fixed in 4% polyformaldehyde. Fixed brain tissues were cut into transverse sections 5 µm thick. The sections were collected on poly-l-lysine-coated slides and air-dried. After blocking, sections were incubated with ab50483 in PBS for 2 hours at 37 °C in a humidified chamber. After washing, slides were incubated with the secondary antibody, a rabbit anti-goat IgG conjugated with horseradish peroxidase for 1 hour at 37 °C in a humidity box.

    References for Anti-alpha+beta SNAP antibody (ab50483)

    This product has been referenced in:
    • Yu Y  et al. Evaluation of blastomere biopsy using mouse model indicates the potential high-risk of neurodegenerative disorders in the offspring. Mol Cell Proteomics : (2009). WB, IHC-FoFr ; Mouse . Read more (PubMed: 19279043) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    IHC - Wholemount
    Sample
    Fruit fly (Drosophila melanogaster) Tissue (Third instar larva)
    Specification
    Third instar larva
    Username

    Dr. Jacob Krans

    Verified customer

    Submitted May 04 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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