The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionKinases that recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.
Sequence similaritiesBelongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily. Contains 1 alpha-type protein kinase domain. Contains 2 Ig-like (immunoglobulin-like) domains.
Immunohistochemistry analysis of paraffin-embedded Human heart tissue, using ab111284 at a dilution of 1/50. left panel shows staining without immunizing peptide right panel shows staining with immunizing peptide
References for Anti-ALPK2 antibody (ab111284)
has not yet been referenced specifically in any publications.
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