Anti-ALS antibody (ab85222)


  • Product nameAnti-ALS antibody
    See all ALS primary antibodies
  • Description
    Rabbit polyclonal to ALS
  • Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide selected from the center region of Human ALS, conjugated to KLH (NP_004961).

  • Positive control
    • Jurkat cell line and mouse spleen tissue lysates. IF/ICC: HeLa cell line.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Purification notesab85222 is prepared by Saturated Ammonium Sulphate (SAS) precipitation followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab85222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/50 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.


  • FunctionInvolved in protein-protein interactions that result in protein complexes, receptor-ligand binding or cell adhesion.
  • Tissue specificityPlasma.
  • Sequence similaritiesContains 19 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Albs antibody
    • ALS antibody
    • ALS_HUMAN antibody
    • IGFALS antibody
    • Insulin like growth factor binding protein acid labile subunit antibody
    • Insulin like growth factor binding protein complex acid labile chain antibody
    • Insulin-like growth factor-binding protein complex acid labile subunit antibody
    • KIAA4111 antibody
    • MGC150140 antibody
    • MGC150141 antibody
    see all

Anti-ALS antibody images

  • ICC/IF image of ab85222 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85222, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC-P human hepatocarcinoma reacted with ab85222, which was peroxidase-conjugated to the secondary ab, followed by DAB staining.

  • Anti-ALS antibody (ab85222) at 1/50 dilution + Jurkat cell line lysate at 35 µg

    Predicted band size : 66 kDa
    Observed band size : 66 kDa
  • All lanes : Anti-ALS antibody (ab85222) at 1/1000 dilution

    Lane 1 : mouse spleen tissue
    Lane 2 : Jurkat cell line
    Lane 3 : mouse liver tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size : 66 kDa
  • Anti-ALS antibody (ab85222) at 1/50 dilution + mouse spleen tissue lysate at 35 µg

    Predicted band size : 66 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)
  • ab85222 flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References for Anti-ALS antibody (ab85222)

ab85222 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: HIER Tris/HCL pH 9.0
Sample Human Tissue sections (human liver)
Specification human liver
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Aug 08 2014

Thank you for contacting us.

We unfortunately do not have alternative product available. I would suggest waiting for ab85222 to be in stock again

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