Overview

  • Product nameAnti-Alx1 antibody
    See all Alx1 primary antibodies
  • Description
    Mouse monoclonal to Alx1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 198-306 of Human Alx1 (NP_008913) with a tag of 26kDa.

  • Positive control
    • Hela nuclear lysate. Recombinant tagged Human Alx1 fragment.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged Alx1 is approximately 3ng/ml if used as a capture antibody.

Target

  • FunctionTranscriptional activator that acts at a palindromic recognition sequence to enhance the activity of the SV40 and TK promoters. Functions as a repressor with the prolactin promoter in vivo. May play a role in chondrocyte differentiation and may also influence cervix development.
  • Tissue specificityCartilage and cervix tissue.
  • Involvement in diseaseDefects in ALX1 are the cause of frontonasal dysplasia type 3 (FND3) [MIM:613456]. The term frontonasal dysplasia describes an array of abnormalities affecting the eyes, forehead and nose and linked to midfacial dysraphia. The clinical picture is highly variable. Major findings include true ocular hypertelorism; broadening of the nasal root; median facial cleft affecting the nose and/or upper lip and palate; unilateral or bilateral clefting of the alae nasi; lack of formation of the nasal tip; anterior cranium bifidum occultum; a V-shaped or widow's peak frontal hairline.
  • Sequence similaritiesBelongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Acetylated at Lys-131 by EP300, leading to increased interaction with EP300 and enhances transcriptional activation activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALX homeobox 1 antibody
    • ALX homeobox protein 1 antibody
    • ALX1 antibody
    • ALX1_HUMAN antibody
    • CART 1 antibody
    • CART-1 antibody
    • CART1 antibody
    • Cartilage homeoprotein 1 antibody
    • Cartilage paired class homeoprotein 1 antibody
    • FND3 antibody
    see all

Anti-Alx1 antibody images

  • Anti-Alx1 antibody (ab77443) at 1 µg/ml + Hela nuclear lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Anti-Alx1 antibody (ab77443) at 1 µg/ml + Recombinant tagged Human Alx1 fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 37 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-Alx1 antibody (ab77443)

ab77443 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"