Overview

  • Product name
    Anti-Alx1 antibody [EPR11331]
    See all Alx1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11331] to Alx1
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Alx1 aa 50-150. The exact sequence is proprietary.
    Database link: Q15699

  • Positive control
    • MCF7, HeLa and HepG2 cell lysate. MCF-7 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
Flow Cyt 1/230. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Transcriptional activator that acts at a palindromic recognition sequence to enhance the activity of the SV40 and TK promoters. Functions as a repressor with the prolactin promoter in vivo. May play a role in chondrocyte differentiation and may also influence cervix development.
  • Tissue specificity
    Cartilage and cervix tissue.
  • Involvement in disease
    Defects in ALX1 are the cause of frontonasal dysplasia type 3 (FND3) [MIM:613456]. The term frontonasal dysplasia describes an array of abnormalities affecting the eyes, forehead and nose and linked to midfacial dysraphia. The clinical picture is highly variable. Major findings include true ocular hypertelorism; broadening of the nasal root; median facial cleft affecting the nose and/or upper lip and palate; unilateral or bilateral clefting of the alae nasi; lack of formation of the nasal tip; anterior cranium bifidum occultum; a V-shaped or widow's peak frontal hairline.
  • Sequence similarities
    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Acetylated at Lys-131 by EP300, leading to increased interaction with EP300 and enhances transcriptional activation activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALX homeobox 1 antibody
    • ALX homeobox protein 1 antibody
    • ALX1 antibody
    • ALX1_HUMAN antibody
    • CART 1 antibody
    • CART-1 antibody
    • CART1 antibody
    • Cartilage homeoprotein 1 antibody
    • Cartilage paired class homeoprotein 1 antibody
    • FND3 antibody
    see all

Images

  • All lanes : Anti-Alx1 antibody [EPR11331] (ab181101) at 1/2000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 36 kDa
  • Flow cytometry analysis of 2% paraformaldehyde fixed MCF7 cells labeling Alx1 with ab181101 at 1/230 dilution (red) or a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at a 1/50 dilution.

  • Anti-Alx1 antibody [EPR11331] (ab181101) at 1/10000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 36 kDa

References

This product has been referenced in:
  • Blue EK  et al. Epigenetic Regulation of Placenta-Specific 8 Contributes to Altered Function of Endothelial Colony-Forming Cells Exposed to Intrauterine Gestational Diabetes Mellitus. Diabetes 64:2664-75 (2015). Read more (PubMed: 25720387) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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