Overview

  • Product name
  • Description
    Rabbit polyclonal to ALX3
  • Specificity
    Detects endogenous levels of total ALX3 protein.
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from internal of human ALX3.

  • Positive control
    • 293 cell extract and HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab64985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • Relevance
    ALX3 (aristaless-like homeobox 3) is a nuclear protein with a homeobox DNA-binding domain. It functions as a transcriptional regulator with a possible role in patterning of the mesoderm during development. Preferential methylation of the ALX3 promoter is associated with advanced-stage neuroblastoma tumors.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • ALX 3 antibody
    • ALX3 homeobox 3 antibody
    • Aristaless like homeobox 3 antibody
    • FND1 antibody
    • Homeobox protein aristaless like 3 antibody
    • MGC138212 antibody
    • MGC141988 antibody
    • Proline rich transcription factor ALX3 antibody
    see all

Anti-ALX3 antibody images

  • All lanes : Anti-ALX3 antibody (ab64985) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HeLa cells, using ab64985 at 1/500-1/1000 dilution. Right hand panel was also treated with immunising peptide.

References for Anti-ALX3 antibody (ab64985)

ab64985 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue (Skin tissue)
Permeabilization
No
Specification
Skin tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
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Submitted May 29 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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