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Rabbit polyclonal to ALX3
Detects endogenous levels of total ALX3 protein.
Predicted to work with:
Synthetic peptide from internal of human ALX3.
293 cell extract and HeLa cells
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
1/500 - 1/1000.
ALX3 (aristaless-like homeobox 3) is a nuclear protein with a homeobox DNA-binding domain. It functions as a transcriptional regulator with a possible role in patterning of the mesoderm during development. Preferential methylation of the ALX3 promoter is associated with advanced-stage neuroblastoma tumors.
ALX 3 antibody
ALX3 homeobox 3 antibody
Aristaless like homeobox 3 antibody
Western blot - Anti-ALX3 antibody (ab64985)
All lanes : Anti-ALX3 antibody (ab64985) at 1/500 dilution Lane 1 : 293 cell extract Lane 2 : 293 cell extract with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size: 37 kDa Observed band size: 37 kDa Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-ALX3 antibody (ab64985)
Immunofluorescence analysis of HeLa cells, using ab64985 at 1/500-1/1000 dilution. Right hand panel was also treated with immunising peptide.
has not yet been referenced specifically in any publications.
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