Recombinant Anti-AMBN antibody [EPR8317] (ab155950)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8317] to AMBN
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AMBN antibody [EPR8317]
See all AMBN primary antibodies -
Description
Rabbit monoclonal [EPR8317] to AMBN -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant AMBN protein
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8317 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
Target
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Function
Involved in the mineralization and structural organization of enamel. -
Tissue specificity
Ameloblast-specific. Located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel. -
Sequence similarities
Belongs to the ameloblastin family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 258 Human
- Omim: 601259 Human
- SwissProt: Q9NP70 Human
- Unigene: 272396 Human
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Alternative names
- AMBN antibody
- AMBN_HUMAN antibody
- Ameloblastin (enamel matrix protein) antibody
- Ameloblastin antibody
Images
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Lane 1 : Anti-AMBN antibody [EPR8317] (ab155950) at 1/1000 dilution
Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
All lanes : Recombinant AMBN protein
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: Lane 1: 1 second. Lane 2: 20 seconds.
AMBN will migrate to higher MW size (PMID: 23782691), the MW is consistent with literature (PMID: 26070558).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155950 has not yet been referenced specifically in any publications.