Overview

  • Product nameAnti-AMBRA1 antibody
    See all AMBRA1 primary antibodies
  • Description
    Rabbit polyclonal to AMBRA1
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the carboxy terminus of human AMBRA1

  • Positive control
    • 3T3 (NIH) cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).

Target

  • FunctionRegulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.
  • Sequence similaritiesContains 3 WD repeats.
  • Cellular localizationCytoplasmic vesicle > autophagosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating molecule in beclin 1 regulated autophagy antibody
    • Activating molecule in BECN1 regulated autophagy protein 1 antibody
    • Activating molecule in BECN1-regulated autophagy protein 1 antibody
    • Ambra1 antibody
    • AMRA1_HUMAN antibody
    • FLJ20294 antibody
    • Hypothetical protein FLJ20294 antibody
    • Hypothetical protein LOC55626 antibody
    • KIAA1736 antibody
    • MGC33725 antibody
    • WDR94 antibody
    see all

Anti-AMBRA1 antibody images

  • Anti-AMBRA1 antibody (ab69501) at 1 µg/ml + 3T3 cell lysate at 15 µg

    Secondary
    Anti-rabbit

    Predicted band size : 143 kDa
    Observed band size : 143 kDa
  • ab69501 at 5 µg/ml staining Ambra1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • Immunofluorescence of Ambra1 in Human Brain cells using ab69501 at 20 ug/ml.

References for Anti-AMBRA1 antibody (ab69501)

ab69501 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Submitted Nov 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"