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The Abcam AMCA Fast Conjugation Kit (ab195224) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides and other biomolecules with AMCA with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.
AMCA (aminomethylcoumarin acetate) is a blue fluorescent dye commonly used in fluorescence microscopy, arrays and in-situ hybridisation. It has an excitation wavelength of 345-350nm and an emission wavelength of 440-460nm.
Unlike standard AMCA labeling procedures, where a large molar excess of AMCA is employed, the Abcam AMCA Fast Conjugation Kit (ab195224) combines a low molar ratio of AMCA:antibody and high labeling efficiency to eliminate the need for purification steps.
The kit comes in 3 sizes for labelling recommended amounts of antibody:
300 μg containing 3 reactions (each for 100 - 200 μg)
1 mg containing 1 reaction (for 1 - 2 mg)
30 μg containing 3 reactions (each for 10 - 20 μg)
The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.
|Components||30 µg||300 µg||1 mg|
|AMCA Conjugation Mix||3 vials||3 vials||1 vial|
|AMCA Modifer Reagent||1 vial||1 vial||1 vial|
|AMCA Quencher Reagent||1 vial||1 vial||1 vial|
Our Abpromise guarantee covers the use of ab195224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent concentration.|
ab195224 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"