Overview

  • Product name
    AMCA Fast Conjugation Kit
  • Product overview

    The Abcam AMCA Fast Conjugation Kit (ab195224) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides and other biomolecules with AMCA with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.

    AMCA (aminomethylcoumarin acetate) is a blue fluorescent dye commonly used in fluorescence microscopy, arrays and in-situ hybridisation. It has an excitation wavelength of 345-350nm and an emission wavelength of 440-460nm.

    Unlike standard AMCA labeling procedures, where a large molar excess of AMCA is employed, the Abcam AMCA Fast Conjugation Kit (ab195224) combines a low molar ratio of AMCA:antibody and high labeling efficiency to eliminate the need for purification steps.

  • Notes

    The kit comes in 3 sizes for labelling recommended amounts of antibody:

    300 μg containing 3 reactions (each for 100 - 200 μg)

    1 mg containing 1 reaction (for 1 - 2 mg)

    30 μg containing 3 reactions (each for 10 - 20 μg)

     

    The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab195224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Images

Protocols

References

ab195224 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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