Overview

  • Product name
    AMCA Fast Conjugation Kit
  • Product overview

    The Abcam AMCA Fast Conjugation Kit (ab195224) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides and other biomolecules with AMCA with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.

    AMCA (aminomethylcoumarin acetate) is a blue fluorescent dye commonly used in fluorescence microscopy, arrays and in-situ hybridisation. It has an excitation wavelength of 345-350nm and an emission wavelength of 440-460nm.

    Unlike standard AMCA labeling procedures, where a large molar excess of AMCA is employed, the Abcam AMCA Fast Conjugation Kit (ab195224) combines a low molar ratio of AMCA:antibody and high labeling efficiency to eliminate the need for purification steps.

  • Notes

    The kit comes in 3 sizes for labelling recommended amounts of antibody:

    300 μg containing 3 reactions (each for 100 - 200 μg)

    1 mg containing 1 reaction (for 1 - 2 mg)

    30 μg containing 3 reactions (each for 10 - 20 μg)

     

    The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab195224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:
  • Boopathy GTK  et al. Cavin-2 regulates the activity and stability of endothelial nitric-oxide synthase (eNOS) in angiogenesis. J Biol Chem 292:17760-17776 (2017). Read more (PubMed: 28912276) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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