Overview

  • Product nameAnti-AMCase antibody
    See all AMCase primary antibodies
  • Description
    Rabbit polyclonal to AMCase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human AMCase aa 108-157 (internal sequence). Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (GSWEGYTGEN SPLYKYPTDT GSNAYLNVDY VMNYWKDNGA PAEKLIVGFP) of Human CHIA (NP_068569).
    Sequence:

    GSWEGYTGENSPLYKYPTDTGSNAYLNVDYVMNYWKDNGAPAEKLIVGFP

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionDegrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.
  • Tissue specificityDetected in lung epithelial cells from asthma patients (at protein level). Highly expressed in stomach. Detected at lower levels in lung.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
    Contains 1 chitin-binding type-2 domain.
  • Cellular localizationCytoplasm and Secreted. Secretion depends on EGFR activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acidic mammalian chitinase [Precursor] antibody
    • Acidic mammalian chitinase antibody
    • AMCase antibody
    • Chia antibody
    • CHIA_HUMAN antibody
    • CHIT2 antibody
    • chitinase, acidic antibody
    • DKFZp313J1722 antibody
    • ECF L antibody
    • Lung specific protein TSA1902 antibody
    • Lung-specific protein TSA1902 antibody
    • RP5 1125M8.1 antibody
    • TSA1902 antibody
    see all

Anti-AMCase antibody images

  • Anti-AMCase antibody (ab83187) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa (possible isoform).
    Gel concentration: 12%

References for Anti-AMCase antibody (ab83187)

ab83187 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"