Human Predicted to work with:
Mouse, Rat, Horse, Chicken, Cow, Cat, Dog, Pig
Synthetic peptide corresponding to Human AMCase aa 108-157 (internal sequence). Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (GSWEGYTGEN SPLYKYPTDT GSNAYLNVDY VMNYWKDNGA PAEKLIVGFP) of Human CHIA (NP_068569). Sequence:
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionDegrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.
Tissue specificityDetected in lung epithelial cells from asthma patients (at protein level). Highly expressed in stomach. Detected at lower levels in lung.
Sequence similaritiesBelongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. Contains 1 chitin-binding type-2 domain.
Cellular localizationCytoplasm and Secreted. Secretion depends on EGFR activity.