Anti-AMACR antibody (ab231333)
Key features and details
- Rabbit polyclonal to AMACR
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-AMACR antibody
See all AMACR primary antibodies -
Description
Rabbit polyclonal to AMACR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human AMACR aa 1-150. Expressed in E.coli. N-terminal tags.
Database link: Q9UHK6 -
Positive control
- WB: Recombinant human AMACR; Pig kidney and liver lysates. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 20 µg/ml. Predicted molecular weight: 42 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 20 µg/ml. Predicted molecular weight: 42 kDa. |
Target
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Function
Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. -
Pathway
Lipid metabolism; bile acid biosynthesis.
Lipid metabolism; fatty acid metabolism. -
Involvement in disease
Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) [MIM:614307]. AMACRD results in elevated plasma concentrations of pristanic acid C27-bile-acid intermediates. It can be associated with polyneuropathy, retinitis pigmentosa, epilepsy.
Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4) [MIM:214950]; also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile. -
Sequence similarities
Belongs to the CaiB/BaiF CoA-transferase family. -
Cellular localization
Peroxisome. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 23600 Human
- Omim: 604489 Human
- SwissProt: Q9UHK6 Human
- Unigene: 508343 Human
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Alternative names
- 2 arylpropionyl CoA epimerase antibody
- 2 methylacyl CoA racemase antibody
- 2-methylacyl-CoA racemase antibody
see all
Images
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Formalin-fixed, paraffin-embedded human kidney tissue stained for AMACR using ab231333 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-AMACR antibody (ab231333) at 2 µg/ml + Pig kidney lysate
Predicted band size: 42 kDa -
Anti-AMACR antibody (ab231333) at 2 µg/ml + Pig liver lysate
Predicted band size: 42 kDa -
Anti-AMACR antibody (ab231333) at 2 µg/ml + Recombinant human AMACR protein
Predicted band size: 42 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231333 has not yet been referenced specifically in any publications.