Overview

  • Product nameAnti-AMD1 antibody
    See all AMD1 primary antibodies
  • Description
    Chicken polyclonal to AMD1
  • SpecificityCatalytic Activity: S-adenosyl-L-methionine = (5-deoxy-5-adenosyl)(3-aminopropyl)-methylsulfonium salt + CO2. Cofactor: Pyruvoyl group. Enzyme Regulation: Both proenzyme processing and catalytic activity are stimulated by putrescine. Catalytic activity is inhibited by iodoacetic acid. Pathway: Amine and polyamine biosynthesis. Subunit: Heterotetramer of two alpha and two beta chains. Similarity: Belongs to the eukaryotic AdoMetDC family. Pathways: KEGG pathway: Arginine and proline metabolism 00330 Protein Functions: Monomeric Gtp-Binding Proteins.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide:

    LARD YSGFDSIQSF FYSRKNFMKP

    , corresponding to N terminal amino acids 96-334 of Human AMD1. Chromosome location: 6q21-q22.

Properties

Applications

Our Abpromise guarantee covers the use of ab37387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/2000. Predicted molecular weight: 38.3 kDa.

Target

  • PathwayAmine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.
  • Sequence similaritiesBelongs to the eukaryotic AdoMetDC family.
  • Post-translational
    modifications
    Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosylmethionine decarboxylase 1 antibody
    • AdoMetDC antibody
    • AMD1 antibody
    • DCAM_HUMAN antibody
    • EC 4.1.1.50 antibody
    • FLJ26964 antibody
    • S adenosylmethionine decarboxylase 1 antibody
    • S adenosylmethionine decarboxylase proenzyme antibody
    • S-adenosylmethionine decarboxylase beta chain antibody
    • SAMDC antibody
    see all

Anti-AMD1 antibody images

  • Direct ELISA of test antigen (5 ug/ml) using ab37387 as primary antibody and Goat anti-IgY HRP as secondary antibody.
  • Anti-AMD1 antibody (ab37387) at 1/2000 dilution (E coli-derived fusion protein)

    Secondary
    Goat anti-IgY-HRP at 1/1000 dilution

    Predicted band size : 38.3 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-AMD1 antibody (ab37387)

ab37387 has not yet been referenced specifically in any publications.

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