• Product name
  • Description
    Rabbit polyclonal to AMD1
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence at the C terminus of human AMD1, identical to the related mouse and rat sequence.

  • Positive control
    • IHC-P: Mammary cancer sections WB: Rat kidney tissue lysis



Our Abpromise guarantee covers the use of ab65820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 38 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
ICC Use a concentration of 2 - 3 µg/ml.


  • Pathway
    Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.
  • Sequence similarities
    Belongs to the eukaryotic AdoMetDC family.
  • Post-translational
    Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosylmethionine decarboxylase 1 antibody
    • AdoMetDC antibody
    • AMD1 antibody
    • DCAM_HUMAN antibody
    • EC antibody
    • FLJ26964 antibody
    • S adenosylmethionine decarboxylase 1 antibody
    • S adenosylmethionine decarboxylase proenzyme antibody
    • S-adenosylmethionine decarboxylase beta chain antibody
    • SAMDC antibody
    see all

Anti-AMD1 antibody images

  • ab65820, at 0.5µg/ml, staining AMD1 in the cytoplasm of formalin fixed and paraffin embedded mammary cancer tissue sections.
  • Anti-AMD1 antibody (ab65820) at 1 µg/ml + Rat kidney tissue lysis

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab65820 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65820 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-AMD1 antibody (ab65820)

This product has been referenced in:
  • Dos Santos Ramos R  et al. Regulation of the polyamine metabolic pathway in the endometrium of cows during early diestrus. Mol Reprod Dev N/A:N/A (2014). Cow . Read more (PubMed: 24659573) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab65820.
Please use the links above to contact us or submit feedback about this product.


Sign up