Overview

  • Product nameAnti-AMD1 antibody
    See all AMD1 primary antibodies
  • Description
    Rabbit polyclonal to AMD1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence at the C terminus of human AMD1, identical to the related mouse and rat sequence.

  • Positive control
    • IHC-P: Mammary cancer sections WB: Rat kidney tissue lysis

Properties

Applications

Our Abpromise guarantee covers the use of ab65820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 38 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
ICC Use a concentration of 2 - 3 µg/ml.

Target

  • PathwayAmine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.
  • Sequence similaritiesBelongs to the eukaryotic AdoMetDC family.
  • Post-translational
    modifications
    Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosylmethionine decarboxylase 1 antibody
    • AdoMetDC antibody
    • AMD1 antibody
    • DCAM_HUMAN antibody
    • EC 4.1.1.50 antibody
    • FLJ26964 antibody
    • S adenosylmethionine decarboxylase 1 antibody
    • S adenosylmethionine decarboxylase proenzyme antibody
    • S-adenosylmethionine decarboxylase beta chain antibody
    • SAMDC antibody
    see all

Anti-AMD1 antibody images

  • ab65820, at 0.5µg/ml, staining AMD1 in the cytoplasm of formalin fixed and paraffin embedded mammary cancer tissue sections.
  • Anti-AMD1 antibody (ab65820) at 1 µg/ml + Rat kidney tissue lysis

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab65820 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65820 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-AMD1 antibody (ab65820)

This product has been referenced in:
  • Dos Santos Ramos R  et al. Regulation of the polyamine metabolic pathway in the endometrium of cows during early diestrus. Mol Reprod Dev N/A:N/A (2014). Cow . Read more (PubMed: 24659573) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"