Overview

  • Product nameAnti-AMD1 antibody
    See all AMD1 primary antibodies
  • Description
    Rabbit polyclonal to AMD1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (GRMNSDCWYL YTLDFPESRV ISQPDQTLEI LMSELDPAVM DQFYMKDGVT) of human AMD1 (NP_001028231; Q5VXN4).

  • Positive control
    • THP1 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. 38 kDa for isoform 1 and 21 kDa for isoform 2. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • PathwayAmine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.
  • Sequence similaritiesBelongs to the eukaryotic AdoMetDC family.
  • Post-translational
    modifications
    Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosylmethionine decarboxylase 1 antibody
    • AdoMetDC antibody
    • AMD1 antibody
    • DCAM_HUMAN antibody
    • EC 4.1.1.50 antibody
    • FLJ26964 antibody
    • S adenosylmethionine decarboxylase 1 antibody
    • S adenosylmethionine decarboxylase proenzyme antibody
    • S-adenosylmethionine decarboxylase beta chain antibody
    • SAMDC antibody
    see all

Anti-AMD1 antibody images

  • Anti-AMD1 antibody (ab87661) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 46 kDa,70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-AMD1 antibody (ab87661)

ab87661 has not yet been referenced specifically in any publications.

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