Overview

  • Product nameAnti-Amelotin antibody
    See all Amelotin primary antibodies
  • Description
    Rabbit polyclonal to Amelotin
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 16-110 (SLPQLKPALG LPPTKLAPDQ GTLPNQQQSN QVFPSLSLIP LTQMLTLGPD LHLLNPAAGM TPGTQTHPLT LGGLNVQQQL HPHVLPIFVT QLGAQ) of Human Amelotin.

  • Positive control
    • Human cerebellum tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-Amelotin antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122312 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122312, at 1/150, staining Amelotin in paraffin embedded Human cerebellum tissue by Immunohistochemistry.

References for Anti-Amelotin antibody (ab122312)

This product has been referenced in:
  • Lee HS  et al. Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans. PLoS One 8:e84201 (2013). IHC-P ; Human . Read more (PubMed: 24376796) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (follicle)
Specification follicle
Permeabilization No
Fixative Paraformaldehyde
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Submitted Apr 02 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (tooth)
Specification tooth
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"