Overview

  • Product name
  • Description
    Rabbit polyclonal to AMELX
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-191 of Human AMELX (UniProt ID: Q99217).

  • Positive control
    • WB: Jurkat and NIH/3T3 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 29089903

Target

  • Function
    Plays a role in biomineralization. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel.
  • Involvement in disease
    Defects in AMELX are the cause of amelogenesis imperfecta hypoplastic type 1 (AIH1) [MIM:301200]. AIH1 is a X-linked defect of dental enamel formation. Teeth have only a thin layer of enamel with normal hardness. The thinness of the enamel makes the teeth appear small.
  • Sequence similarities
    Belongs to the amelogenin family.
  • Developmental stage
    Transiently but abundantly expressed by ameloblasts during tooth development. Amelogenin is the predominant protein in developing dental enamel.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI1E antibody
    • AIH1 antibody
    • ALGN antibody
    • Amel antibody
    • Amelogenesis imperfecta 1 antibody
    • Amelogenin (amelogenesis imperfecta 1, X linked) antibody
    • Amelogenin (X chromosome) antibody
    • Amelogenin (X chromosome, amelogenesis imperfecta 1) antibody
    • Amelogenin antibody
    • Amelogenin X isoform antibody
    • Amelogenin, X linked antibody
    • AMELX antibody
    • AMELX_HUMAN antibody
    • Amg antibody
    • AMGL antibody
    • AMGX antibody
    • OTTHUMP00000022906 antibody
    • OTTHUMP00000022907 antibody
    • X isoform antibody
    see all

Images

  • Anti-AMELX antibody (ab153915) at 1/500 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 22 kDa



    12% SDS PAGE
  • Anti-AMELX antibody (ab153915) at 1/500 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 22 kDa



    12% SDS PAGE

References

This product has been referenced in:
  • Pandya M  et al. Intravesicular Phosphatase PHOSPHO1 Function in Enamel Mineralization and Prism Formation. Front Physiol 8:805 (2017). IHC-P ; Mouse . Read more (PubMed: 29089903) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Newborn mouse head, ameloblasts and enamel)
Antigen retrieval step
None
Permeabilization
No
Specification
Newborn mouse head, ameloblasts and enamel
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Mr. Pierfrancesco Pagella

Verified customer

Submitted Jul 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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