Overview

  • Product nameAnti-AMFR antibody
    See all AMFR primary antibodies
  • Description
    Rabbit polyclonal to AMFR
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 500 and 550 of Human AMFR (NP_001135.3).

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab101284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionE3 ubiquitin-protein ligase which can target both itself and other proteins including CD3D and APOB for proteasomal degradation. Mediates polyubiquitination of CYP3A4. Specific receptor for the autocrine motility factor. Mediates tumor invasion and metastasis. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Promotes ubiquitination of misfolded proteins such as mutant CFTR; proposed to mediate sequential ubiquitination by recognizing already ubiquitin-conjugated substrates and to cooperate with E3 ubiquitin-protein ligase RNF5.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 CUE domain.
    Contains 1 RING-type zinc finger.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMF receptor antibody
    • AMF receptor isoform 1 antibody
    • AMF receptor isoform 2 antibody
    • AMFR antibody
    • AMFR2_HUMAN antibody
    • Autocrine motility factor receptor antibody
    • Autocrine motility factor receptor precursor, isoform 1 antibody
    • Autocrine motility factor receptor precursor, isoform 2 antibody
    • E3 ubiquitin-protein ligase AMFR antibody
    • gp78 antibody
    • isoform 2 antibody
    • RING finger protein 45 antibody
    • RNF 45 antibody
    • RNF45 antibody
    see all

Anti-AMFR antibody images

  • All lanes : Anti-AMFR antibody (ab101284) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 73 kDa


    Exposure time : 3 minutes
  • Detection of Human AMFR in immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab101284 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent Western blotting.

    Detection by chemiluminescence with an exposure time of 30 seconds.

References for Anti-AMFR antibody (ab101284)

ab101284 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"