Overview

  • Product nameAnti-AMFR antibody
    See all AMFR primary antibodies
  • Description
    Mouse monoclonal to AMFR
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: QASNSQLNAM AHQIQEMFPQ VPYHLVLQDL QLTRSVEITT DNILEGRIQV PFPTQRSDSI RPALNSPVER PSSDQEEGET SAQTERVPLD LSPRLEETLD , corresponding to amino acids 451-551 of Human AMFR

Properties

Applications

Our Abpromise guarantee covers the use of ab54787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionE3 ubiquitin-protein ligase which can target both itself and other proteins including CD3D and APOB for proteasomal degradation. Mediates polyubiquitination of CYP3A4. Specific receptor for the autocrine motility factor. Mediates tumor invasion and metastasis. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Promotes ubiquitination of misfolded proteins such as mutant CFTR; proposed to mediate sequential ubiquitination by recognizing already ubiquitin-conjugated substrates and to cooperate with E3 ubiquitin-protein ligase RNF5.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesContains 1 CUE domain.
      Contains 1 RING-type zinc finger.
    • Cellular localizationEndoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AMF receptor antibody
      • AMF receptor isoform 1 antibody
      • AMF receptor isoform 2 antibody
      • AMFR antibody
      • AMFR2_HUMAN antibody
      • Autocrine motility factor receptor antibody
      • Autocrine motility factor receptor precursor, isoform 1 antibody
      • Autocrine motility factor receptor precursor, isoform 2 antibody
      • E3 ubiquitin-protein ligase AMFR antibody
      • gp78 antibody
      • isoform 2 antibody
      • RING finger protein 45 antibody
      • RNF 45 antibody
      • RNF45 antibody
      see all

    Anti-AMFR antibody images

    • Western blot against tagged recombinant protein immunogen using ab54787 AMFR antibody at 1ug/ml. Predicted band size of recombinant protein is 37 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    References for Anti-AMFR antibody (ab54787)

    This product has been referenced in:
    • Joshi B  et al. A role for KAI1 in promotion of cell proliferation and mammary gland hyperplasia by the gp78 ubiquitin ligase. J Biol Chem 285:8830-9 (2010). WB ; Mouse . Read more (PubMed: 20089858) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293)
    Loading amount 35 µg
    Specification HEK293
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.05µg/mL · Temperature: 20°C
    Username

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    Verified customer

    Submitted Aug 03 2012

    DISCOUNT CODE: ***

    Expiration date: September 30th, 2012

    I am very pleased to hear you would like to accept our offer and test ab54787 in human. The abovecode will give you: 1 free primary antibody before the expiration date and i...

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    Thank you for contacting us.

    While we do not have any remaining stock of lot ******, we do have a new lot available. This antibody has not been validated for use on endogenous protein and has only been tested against the recombinant immunogen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"