The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/20 - 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium Citrate buffer pH 6.0 is recommended for this purpose.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceAnti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.
Ab24542, at a dilution of 1/20 to 1/40, staining formalin fixed paraffin embedded ovary tissue from a 25 day old mouse by Immunohystochemistry.
References for Anti-AMH antibody [5/6] (ab24542)
This product has been referenced in:
Kevenaar ME et al. Serum anti-mullerian hormone levels reflect the size of the primordial follicle pool in mice. Endocrinology147:3228-34 (2006).
Read more (PubMed: 16556768) »
Weenen C et al. Anti-Müllerian hormone expression pattern in the human ovary: potential implications for initial and cyclic follicle recruitment. Mol Hum Reprod10:77-83 (2004).
Read more (PubMed: 14742691) »