Overview

  • Product name
  • Description
    Rabbit polyclonal to AMH
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 430-460 of Human AMH

  • Positive control
    • 293 cell lysate; Mouse liver tissue lysate; Prostate carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab103233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Anti muellerian hormone antibody
    • MIF antibody
    • MIS antibody
    • Muellerian inhibiting factor antibody
    • Mullerian inhibiting substance antibody
    see all

Images

  • Anti-AMH antibody (ab103233) at 1/100 dilution + 293 cell lysate at 35 µg

    Predicted band size : 60 kDa
  • Anti-AMH antibody (ab103233) at 1/100 dilution + Mouse liver tissue lysate at 35 µg

    Predicted band size : 60 kDa
  • ab103233, at 1/50 dilution, staining AMH in formalin-fixed, paraffin-embedded prostate carcinoma by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

References

This product has been referenced in:
  • Del Valle I  et al. A genomic atlas of human adrenal and gonad development. Wellcome Open Res 2:25 (2017). IHC ; Human . Read more (PubMed: 28459107) »
  • Wang W  et al. Overexpression of Uromodulin-like1 accelerates follicle depletion and subsequent ovarian degeneration. Cell Death Dis 3:e433 (2012). WB . Read more (PubMed: 23190605) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Sep 29 2016

Thank you very much for your interest in ab103233.

To our knowledge, ab103233 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab103233 now, test it in ICC/IF and submit feedback to us in the fo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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