Overview

  • Product name
  • Description
    Rabbit polyclonal to AMH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 468-517 (SVDLRAERSV LIPETYQANN CQGVCGWPQS DRNPRYGNHV VLLLKMQARG) of Human AMH (NP_000470).

  • Positive control
    • Human fetal small intestine lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

Images

  • Anti-AMH antibody (ab84952) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal small intestine lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 60 kDa
    Observed band size: 65 kDa (why is the actual band size different from the predicted?)



    Gel concentration: 12%
  • ab84952 staining AMH in human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

This product has been referenced in:
  • Nistal M  et al. Sertoli cell dedifferentiation in human cryptorchidism and gender reassignment shows similarities between fetal environmental and adult medical treatment estrogen and antiandrogen exposure. Reprod Toxicol 42:172-9 (2013). Human . Read more (PubMed: 24012888) »

See 1 Publication for this product

Customer reviews and Q&As

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Application
Western blot
Sample
Rat Cell lysate - whole cell (Testicular Sertoli cell)
Loading amount
10 µg
Specification
Testicular Sertoli cell
Treatment
100nm genistein and/or 100nm daidzein
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Ms. India Napier

Verified customer

Submitted Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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