Overview

  • Product nameAnti-AMHR2 antibody [MIG7]
    See all AMHR2 primary antibodies
  • Description
    Mouse monoclonal [MIG7] to AMHR2
  • SpecificityRecognizes human cells bearing AMHR2 extracellular domain.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide:

    NANYSHLPPPGSPG

    , corresponding to amino acids 117-130 of Human AMHR2

Properties

Applications

Our Abpromise guarantee covers the use of ab64762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
Indirect ELISA Use at an assay dependent concentration.

Target

  • FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone.
  • Involvement in diseaseDefects in AMHR2 are the cause of persistent Muellerian duct syndrome type 2 (PMDS2) [MIM:261550]. PMDS2 is a form of male pseudohermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMH type II receptor antibody
    • AMHR antibody
    • AMHR2 antibody
    • AMHR2_HUMAN antibody
    • Anti Mullerian hormone receptor, type II antibody
    • Anti-Muellerian hormone type II receptor antibody
    • Anti-Muellerian hormone type-2 receptor antibody
    • MGC141312 antibody
    • MIS type II receptor antibody
    • MISR2 antibody
    • MISRII antibody
    • MRII antibody
    • Muellerian hormone type 2 receptor antibody
    • Muellerian hormone type II receptor antibody
    • Muellerian inhibiting substance type II receptor antibody
    • Mullerian hormone receptor type II antibody
    • Mullerian inhibiting substance type II receptor antibody
    see all

Anti-AMHR2 antibody [MIG7] images

  • Overlay histogram showing K562 cells stained with ab64762 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64762, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-AMHR2 antibody [MIG7] (ab64762)

This product has been referenced in:
  • Corbetta S  et al. Gonadal structures in a fetus with complete androgen insensitivity syndrome and persistent Müllerian derivatives: comparison with normal fetal development. Fertil Steril 95:1119.e9-1119.e14 (2011). IHC-P ; Human . Read more (PubMed: 20971460) »

See 1 Publication for this product

Product Wall

We do not have data from tests of bovine samples but BLAST of the immunogen sequence that generated the antibody shows 93% identity with the bovine protein. So, reactivity is likely, though not covered by our standard Abpromise guarantee of a replaceme...

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Merci de nous avoir contactés.

J'ai contacté notre laboratoire qui m’a confirmé que ICC / IF n’a pas été testé pour ab64762. Je vous remercie d'avoir attiré notre attention à...

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Thank you for your inquiry.

I can confirm thatAnti-Müllerian Hormone Receptor is expressed by ovarian, breast, and prostate cancers.

Masiakos PT, et al. Human ovarian cancer, cell lines, and primary ascites cells express the hu...

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Thank you for contacting Abcam regarding ab64762. I have contacted the lab and we have not determined the cross-reactivity of this antibody with TGF beta. I am sorry for the inconvenience. I hope this information is helpful. Please do not hesi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Endometrium)
Specification Endometrium
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step No blocking step used for 15 minute(s) · Temperature: 25°C
Username

Mr. Louie Ye

Verified customer

Submitted Sep 17 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"