Anti-AMID antibody - N-terminal (ab155326)
Key features and details
- Rabbit polyclonal to AMID - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AMID antibody - N-terminal
See all AMID primary antibodies -
Description
Rabbit polyclonal to AMID - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 1-43 of Human AMID (UniProt ID: Q9BRQ8).
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Positive control
- HepG2 and MOLT4 cell lysates; Human gastric carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. |
Target
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Function
Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Binds to DNA in a sequence-independent manner. May contribute to genotoxin-induced growth arrest. -
Tissue specificity
Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expressed following p53/TP53 induction. The shorter 1.8 kb transcript seems to be the major transcript in EB1 colon cancer cells. -
Sequence similarities
Belongs to the FAD-dependent oxidoreductase family. -
Cellular localization
Cytoplasm. Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 84883 Human
- Omim: 605159 Human
- SwissProt: Q9BRQ8 Human
- Unigene: 655377 Human
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Alternative names
- 5430437E11Rik antibody
- aifm2 antibody
- AIFM2_HUMAN antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155326 has not yet been referenced specifically in any publications.