Overview

  • Product name
    Amido black 10B
  • Description
    Histological and protein dye.
  • Alternative names
    • Acid Black 1
    • Buffalo Black NBR
    • Naphthol Blue Black
  • Biological description

    Diazo dye used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. Also utilized in cell proliferation assays and histochemical studies, and as a forensic stain typically used for enhancing latent prints contaminated with blood.

Properties

  • Chemical name
    4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalenedisulfonic acid disodium salt
  • Molecular weight
    616.50
  • Chemical structure
    Chemical Structure
  • Molecular formula
    C22H14N6Na2O9S2
  • CAS Number
    1064-48-8
  • Storage instructions
    Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:
  • Onder S  et al. Decolorization of naphthol blue black using the horseradish peroxidase. Appl Biochem Biotechnol 163:433-43 (2011). Read more (PubMed: 20703828) »
  • Tetlow LC & Woolley DE Histamine stimulates the proliferation of human articular chondrocytes in vitro and is expressed by chondrocytes in osteoarthritic cartilage. Ann Rheum Dis 62:991-4 (2003). Read more (PubMed: 12972479) »
  • Perricone MA  et al. Quantitation of fibroblast population growth rate in situ using computerized image analysis. Microsc Res Tech 31:257-64 (1995). Read more (PubMed: 7670164) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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