Anti-AMIGO2 antibody (ab84416)


  • Product name
  • Description
    Rabbit polyclonal to AMIGO2
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A 15 amino acid peptide from near the C terminal residues of Human AMIGO2 (NM_181847)

  • Positive control
    • Human breast carcinoma tissue.


  • Form
    Lyophilised:Reconstitute in 200ul sterile H2O.
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 2% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab84416 is by a peptide affinity column.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab84416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
ICC/IF 1/100.


  • Function
    Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.
  • Tissue specificity
    Highest levels in breast, ovary, cervix, and uterus. Lower levels in lung, colon, and rectum. Differentially expressed in 56% of thyroid, 57% of pancreatic and 45% of stomach cancers.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. AMIGO family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 6 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Cellular localization
    Cell membrane. Nucleus. Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adhesion molecule with Ig like domain 2 antibody
    • ALI 1 antibody
    • ALI1 antibody
    • Alivin 1 antibody
    • Alivin-1 antibody
    • Alivin1 antibody
    • AMGO2_HUMAN antibody
    • AMIGO 2 antibody
    • AMIGO-2 antibody
    • Amigo2 antibody
    • Amphoterin induced gene 2 antibody
    • Amphoterin induced protein 2 antibody
    • Amphoterin-induced gene and open reading frame 2 antibody
    • Amphoterin-induced protein 2 antibody
    • DEGA antibody
    • Differentially expressed in gastric adenocarcinoma antibody
    • Differentially expressed in gastric adenocarcinomas antibody
    • Transmembrane protein AMIGO 2 antibody
    • Transmembrane protein AMIGO2 antibody
    see all

Anti-AMIGO2 antibody images

  • ab84416 at 1/100 dilution staining AMIGO2 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

References for Anti-AMIGO2 antibody (ab84416)

ab84416 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (melanoma cell line)
Gel Running Conditions
Reduced Denaturing (gradient 4-12% NuPAGE)
Loading amount
30 µg
melanoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 12 2016

Thank you for contacting us.
Your credit note ID is *****.
I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the...

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Thank you for your reply.

I would be happy to raise a discount code for testing ab84416 in Mouse samples with WB if you could please use Human fetal brain lysate or Human breast carcinoma tissue as positive control.

As two untested ...

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Thank you for your reply.

I am happy to hear you are interested in participating in our Abreview system. However, ab84416 has not been tested in WB and unfortunately the discount code is only applicable for only one untested species / applica...

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Thank you for your patience.

Both anti-AMIGO antibodies (ab84416 and ab84476) share the same immunogen sequence. In fact the difference between both antibodies is the purification process. While ab84476 is provided as whole antiserum, ab84416...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (Human umbilical vein endothelial cells)
Human umbilical vein endothelial cells
Yes - triton X-100

Abcam user community

Verified customer

Submitted Oct 21 2011

DISCOUNT CODE: xxxxx Expiration date: 2012-01-06 I am very pleased to hear you would like to accept our offer and test ab84416 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, pleas...

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