The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 21 kDa).
FunctionTranscriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
Tissue specificityFound predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
Sequence similaritiesBelongs to the WD repeat Groucho/TLE family.
DomainLacks the C-terminal WD repeats.
Post-translational modificationsUbiquitinated by XIAP/BIRC4.