Anti-Amino-terminal enhancer of split antibody (ab62555)


  • Product nameAnti-Amino-terminal enhancer of split antibody
    See all Amino-terminal enhancer of split primary antibodies
  • Description
    Rabbit polyclonal to Amino-terminal enhancer of split
  • Tested applicationsSuitable for: ICC, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid peptide from near the carboxy terminus of human GRG (Groucho homologue).

  • Positive control
    • 293 cell lysate. HeLa cells.



Our Abpromise guarantee covers the use of ab62555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use a concentration of 20 µg/ml.


  • FunctionTranscriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
  • Tissue specificityFound predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
  • Sequence similaritiesBelongs to the WD repeat Groucho/TLE family.
  • DomainLacks the C-terminal WD repeats.
  • Post-translational
    Ubiquitinated by XIAP/BIRC4.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aes 1 antibody
    • Aes 2 antibody
    • aes antibody
    • AES_HUMAN antibody
    • Aes1 antibody
    • Aes2 antibody
    • Amino enhancer of split antibody
    • Amino terminal enhancer of split antibody
    • Amino-terminal enhancer of split antibody
    • Esp 1 antibody
    • Esp1 antibody
    • Gp130 associated protein GAM antibody
    • Gp130-associated protein GAM antibody
    • GRG 5 antibody
    • GRG antibody
    • GRG protein antibody
    • Grg-5 antibody
    • GRG5 antibody
    • Groucho-related protein 5 antibody
    • Protein ESP 1 antibody
    • Protein ESP1 antibody
    • Protein GRG antibody
    • TLE 5 antibody
    • TLE5 antibody
    see all

Anti-Amino-terminal enhancer of split antibody images

  • Lane 1 : Anti-Amino-terminal enhancer of split antibody (ab62555) at 1 µg/ml
    Lane 2 : Anti-Amino-terminal enhancer of split antibody (ab62555) at 2 µg/ml
    Lane 3 : Anti-Amino-terminal enhancer of split antibody (ab62555) at 4 µg/ml

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 19 kDa. We are unsure as to the identity of these extra bands.
  • ab62555, at 10µg/ml, staining GRG (Groucho homologue) in HeLa cells by immunocytochemistry.
  • Immunofluorescence of AES in Hela cells using ab62555 at 20 ug/ml.

References for Anti-Amino-terminal enhancer of split antibody (ab62555)

This product has been referenced in:
  • Metzger DE  et al. The transcriptional co-repressor Grg3/Tle3 promotes pancreatic endocrine progenitor delamination and ß-cell differentiation. Development 139:1447-56 (2012). ICC/IF ; Mouse . Read more (PubMed: 22434868) »

See 1 Publication for this product

Product Wall

Thank you for submitting your review of ab62555 last month. This review has now been published, but I would like to follow up with you since the results were below average and we do monitor the quality of all our products. Could you give me some brief...

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (EScell)
Specification EScell
Fixative Paraformaldehyde
Permeabilization Yes - PBS/Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 21 2011

Thank you very much for your interest in ab86016, ab16638 and ab62555. To our knowledge, these antibodies have not been tested in with IF. ab16638, although predicted to react with mouse GRG, this has also not been tested as yet. Therefore, I c...

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