Anti-Amino-terminal enhancer of split antibody (ab62556)

Overview

  • Product name
    Anti-Amino-terminal enhancer of split antibody
    See all Amino-terminal enhancer of split primary antibodies
  • Description
    Rabbit polyclonal to Amino-terminal enhancer of split
  • Tested applications
    Suitable for: ICC, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    16 amino acid synthetic peptide from near the amino-terminus of human GRG (Groucho homologue)

  • Positive control
    • 293 cells and cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function
    Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
  • Tissue specificity
    Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
  • Sequence similarities
    Belongs to the WD repeat Groucho/TLE family.
  • Domain
    Lacks the C-terminal WD repeats.
  • Post-translational
    modifications
    Ubiquitinated by XIAP/BIRC4.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aes 1 antibody
    • Aes 2 antibody
    • aes antibody
    • AES_HUMAN antibody
    • Aes1 antibody
    • Aes2 antibody
    • Amino enhancer of split antibody
    • Amino terminal enhancer of split antibody
    • Amino-terminal enhancer of split antibody
    • Esp 1 antibody
    • Esp1 antibody
    • Gp130 associated protein GAM antibody
    • Gp130-associated protein GAM antibody
    • GRG 5 antibody
    • GRG antibody
    • GRG protein antibody
    • Grg-5 antibody
    • GRG5 antibody
    • Groucho-related protein 5 antibody
    • Protein ESP 1 antibody
    • Protein ESP1 antibody
    • Protein GRG antibody
    • TLE 5 antibody
    • TLE5 antibody
    see all

Images

  • Lane 1 : Anti-Amino-terminal enhancer of split antibody (ab62556) at 2 µg/ml
    Lane 2 : Anti-Amino-terminal enhancer of split antibody (ab62556) at 4 µg/ml

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 22 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa (possible isoform).
  • ab62556 at 10µg/ml staining GRG (Groucho homologue) in 293 cells by Immunocytochemistry.
  • Immunofluorescence of AES in 293 cells using ab62556 at 20 ug/ml.

References

ab62556 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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