Overview

  • Product nameAnti-Aminoacylase 1 antibody
    See all Aminoacylase 1 primary antibodies
  • Description
    Mouse monoclonal to Aminoacylase 1
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-409 of Human Aminoacylase 1

Properties

Applications

Our Abpromise guarantee covers the use of ab54960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IF/ICC: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).
    • Tissue specificityExpression is highest in kidney, strong in brain and weaker in placenta and spleen.
    • Involvement in diseaseDefects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D) [MIM:609924]. ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids.
    • Sequence similaritiesBelongs to the peptidase M20A family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACY 1 antibody
      • ACY-1 antibody
      • Acy1 antibody
      • ACY1_HUMAN antibody
      • ACY1D antibody
      • ACYLASE antibody
      • Acylase I antibody
      • Aminoacylase-1 antibody
      • EC 3.5.1.14 antibody
      • epididymis secretory protein Li 5 antibody
      • HEL-S-5 antibody
      • N acyl L amino acid amidohydrolase antibody
      • N-acyl-L-amino-acid amidohydrolase antibody
      • OTTHUMP00000212459 antibody
      • OTTHUMP00000212462 antibody
      • OTTHUMP00000212463 antibody
      • OTTHUMP00000212464 antibody
      • OTTHUMP00000212465 antibody
      see all

    Anti-Aminoacylase 1 antibody images

    • Aminoacylase 1 antibody (ab54960) used in immunofluorescence at 10ug/ml on HeLa cells.
    • Aminoacylase 1 antibody (ab54960) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B tissue.


    • Predicted band size : 46 kDa
      Aminoacylase 1 antibody (ab54960) at 1ug/lane + K-562 cell lysate at 25ug/lane.

    References for Anti-Aminoacylase 1 antibody (ab54960)

    This product has been referenced in:
    • Wei X  et al. Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma. Cancer Lett 351:117-25 (2014). WB, IHC ; Human . Read more (PubMed: 24846301) »
    • Jin GZ  et al. A novel panel of biomarkers in distinction of small well-differentiated HCC from dysplastic nodules and outcome values. BMC Cancer 13:161 (2013). IHC-P ; Human . Read more (PubMed: 23537217) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab54960.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"