Anti-Aminoadipate aminotransferase antibody (ab59132)

Overview

  • Product name
    Anti-Aminoadipate aminotransferase antibody
    See all Aminoadipate aminotransferase primary antibodies
  • Description
    Rabbit polyclonal to Aminoadipate aminotransferase
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: AGGLPNPNMF PFKTAVITVE NGKTIQFGEE MMKRALQYSP SAGIPELLSW, corresponding to amino acids 37-86 of Human Aminoadipate aminotransferase

  • Positive control
    • HepG2 cells; kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab59132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

Target

  • Function
    Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
  • Tissue specificity
    Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary.
  • Pathway
    Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 4/6.
  • Sequence similarities
    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 aminoadipate aminotransferase antibody
    • 2 aminoadipate transaminase antibody
    • 2-aminoadipate aminotransferase antibody
    • 2-aminoadipate transaminase antibody
    • Aadat antibody
    • AADAT_HUMAN antibody
    • Aadt antibody
    • AI875679 antibody
    • Alpha aminoadipate aminotransferase antibody
    • Alpha-aminoadipate aminotransferase antibody
    • Aminoadipate aminotransferase antibody
    • EC 2.6.1.39 antibody
    • EC 2.6.1.7 antibody
    • KAT/AadAT antibody
    • KAT2 antibody
    • KATII antibody
    • Kynurenine oxoglutarate transaminase 2 antibody
    • Kynurenine aminotransferase II antibody
    • Kynurenine oxoglutarate aminotransferase II antibody
    • Kynurenine oxoglutarate transaminase II antibody
    • Kynurenine--oxoglutarate aminotransferase II antibody
    • Kynurenine--oxoglutarate transaminase II antibody
    • Kynurenine/alpha-aminoadipate aminotransferase mitochondrial [Precursor] antibody
    • Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial antibody
    • L kynurenine/alpha aminoadipate aminotransferase antibody
    see all

Images

  • Anti-Aminoadipate aminotransferase antibody (ab59132) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Aminoadipate aminotransferase with ab59132 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References

ab59132 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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