Anti-Aminoadipate aminotransferase antibody (ab89608)

Overview

  • Product name
    Anti-Aminoadipate aminotransferase antibody
    See all Aminoadipate aminotransferase primary antibodies
  • Description
    Mouse polyclonal to Aminoadipate aminotransferase
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length protein corresponding to amino acids 1-429 of Human Aminoadipate aminotransferase (AAH31068.1).

  • Positive control
    • Human liver lysate, Aminoadipate aminotransferase transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab89608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.

Target

  • Function
    Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
  • Tissue specificity
    Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary.
  • Pathway
    Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 4/6.
  • Sequence similarities
    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 aminoadipate aminotransferase antibody
    • 2 aminoadipate transaminase antibody
    • 2-aminoadipate aminotransferase antibody
    • 2-aminoadipate transaminase antibody
    • Aadat antibody
    • AADAT_HUMAN antibody
    • Aadt antibody
    • AI875679 antibody
    • Alpha aminoadipate aminotransferase antibody
    • Alpha-aminoadipate aminotransferase antibody
    • Aminoadipate aminotransferase antibody
    • EC 2.6.1.39 antibody
    • EC 2.6.1.7 antibody
    • KAT/AadAT antibody
    • KAT2 antibody
    • KATII antibody
    • Kynurenine oxoglutarate transaminase 2 antibody
    • Kynurenine aminotransferase II antibody
    • Kynurenine oxoglutarate aminotransferase II antibody
    • Kynurenine oxoglutarate transaminase II antibody
    • Kynurenine--oxoglutarate aminotransferase II antibody
    • Kynurenine--oxoglutarate transaminase II antibody
    • Kynurenine/alpha-aminoadipate aminotransferase mitochondrial [Precursor] antibody
    • Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial antibody
    • L kynurenine/alpha aminoadipate aminotransferase antibody
    see all

Anti-Aminoadipate aminotransferase antibody images

  • Anti-Aminoadipate aminotransferase antibody (ab89608) at 1 µg/ml + Human liver lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Aminoadipate aminotransferase antibody (ab89608) at 1 µg/ml

    Lane 1 : Aminoadipate aminotransferase transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-Aminoadipate aminotransferase antibody (ab89608)

ab89608 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Sample
Rat Tissue lysate - whole (Hypothalamus)
Specification
Hypothalamus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - other (hippocampus)
Loading amount
20 µg
Specification
hippocampus
Gel Running Conditions
Reduced Denaturing (9)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Jana Aradska

Verified customer

Submitted Aug 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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