Anti-Aminopeptidase A antibody [1C4] (ab119798)

Overview

  • Product nameAnti-Aminopeptidase A antibody [1C4]
    See all Aminopeptidase A primary antibodies
  • Description
    Mouse monoclonal [1C4] to Aminopeptidase A
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Aminopeptidase A. Produced in HEK293T cells.
    Database link: NP_001968

  • Positive control
    • HEK293T cells transfected with Aminopeptidase A, Human Kidney, Carcinoma of Human kidney, Human liver and Human endometrium tissues, COS7 cells transiently transfected with Aminopeptidase A, Hela cells
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab119798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 109 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionAppears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
  • Tissue specificityExpressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
  • Sequence similaritiesBelongs to the peptidase M1 family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aminopeptidase A antibody
    • AMPE_HUMAN antibody
    • AP-A antibody
    • APA antibody
    • bp-1 antibody
    • bp1 antibody
    • CD249 antibody
    • Differentiation antigen gp160 antibody
    • EAP antibody
    • Enpep antibody
    • Glutamyl aminopeptidase (aminopeptidase A) antibody
    • Glutamyl aminopeptidase antibody
    • Gp160 antibody
    • Ly51 antibody
    see all

Anti-Aminopeptidase A antibody [1C4] images

  • All lanes : Anti-Aminopeptidase A antibody [1C4] (ab119798) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY BP1

    Lysates/proteins at 5 µg/ml per lane.


    Predicted band size : 109 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210521)
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue using ab119798 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human Kidney tissue using ab119798 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human liver tissue using ab119798 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human endometrium tissue using ab119798 at a dilution of 1/150.
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BP1 using ab119798 at a dilution of 1/100.
  • HEK293T cells transfected with either RC210521 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119798 at a dilution of 1/100, and then analyzed by flow cytometry.
  • Flow cytometric Analysis of Hela cells, using ab119798 at a dilution of 1/100 (Red), compared to a nonspecific negative control antibody (Blue).

References for Anti-Aminopeptidase A antibody [1C4] (ab119798)

ab119798 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Permeabilization No
Sample Human Cell (hESC H9)
Specification hESC H9
Gating Strategy Doublet discrimination gating/live-dead discrimination (PI)/GFP-expressing cells
Preparation Cell harvesting/tissue preparation method: mild trypsinisation/non-enzymatic dissociation
Sample buffer: FACS staining buffer
Username

Dr. Dmitry Shaposhnikov

Verified customer

Submitted Dec 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"