Overview

  • Product nameAnti-Aminopeptidase A antibody
    See all Aminopeptidase A primary antibodies
  • Description
    Goat polyclonal to Aminopeptidase A
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Aminopeptidase A (internal sequence).
    Sequence:

    EQYQKTSLAQEKEK

  • Positive control
    • Human testis lysate. Human kidney tissue sections.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 109 kDa). The observed molecular weight corresponds to earlier findings in literature, Ando et al. PMID 11994381.

Target

Anti-Aminopeptidase A antibody images

  • Anti-Aminopeptidase A antibody (ab36122) at 1 µg/ml + Human Testis Lysate at 30 µg
    Developed using the ECL technique

    Predicted band size : 109 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
    Primary incubated for 1 hour.
  • ab36122 at 3.8µg/ml staining Aminopeptidase A in human kidney tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, AP-staining. Image shows staining of basement membrane and the mesangium in the glomerulus.

  • ab36122 staining Aminopeptidase A in rat kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate. Samples were then blocked using 20% serum for 1 hour at 25°C and then incubated with ab36122 at 3µg/ml for 1 hour. The secondary used was an undiluted HRP conjugated polyclonal.

    See Abreview

References for Anti-Aminopeptidase A antibody (ab36122)

This product has been referenced in:
  • Dvash E  et al. Leukotriene C4 is the major trigger of stress-induced oxidative DNA damage. Nat Commun 6:10112 (2015). IHC ; Mouse . Read more (PubMed: 26656251) »
  • Härdtner C  et al. High glucose activates the alternative ACE2/Ang-(1-7)/Mas and APN/Ang IV/IRAP RAS axes in pancreatic ß-cells. Int J Mol Med 32:795-804 (2013). WB ; Rat . Read more (PubMed: 23942780) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Dr. Carl Atkinson

Verified customer

Submitted Feb 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"