Recombinant
RabMAb

Anti-Aminopeptidase A antibody [EPR9411(2)] (ab155991)

Overview

  • Product name
    Anti-Aminopeptidase A antibody [EPR9411(2)]
    See all Aminopeptidase A primary antibodies
  • Description
    Rabbit monoclonal [EPR9411(2)] to Aminopeptidase A
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Aminopeptidase A.

  • Positive control
    • Human fetal lung and fetal kidney lysates; Human kidney and placenta tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab155991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 109 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
    • Tissue specificity
      Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
    • Sequence similarities
      Belongs to the peptidase M1 family.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Aminopeptidase A antibody
      • AMPE_HUMAN antibody
      • AP-A antibody
      • APA antibody
      • bp-1 antibody
      • bp1 antibody
      • CD249 antibody
      • Differentiation antigen gp160 antibody
      • EAP antibody
      • Enpep antibody
      • Glutamyl aminopeptidase (aminopeptidase A) antibody
      • Glutamyl aminopeptidase antibody
      • Gp160 antibody
      • Ly51 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Aminopeptidase A with ab155991 at 1/100 dilution.
    • All lanes : Anti-Aminopeptidase A antibody [EPR9411(2)] (ab155991) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : Human fetal kidney lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 109 kDa
    • ab155991 showing +ve staining in Human vascular endothelium.
    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Aminopeptidase A with ab155991 at 1/100 dilution.
    • ab155991 showing -ve staining in Human normal prostate.
    • ab155991 showing -ve staining in Human ovarian carcinoma.
    • ab155991 showing -ve staining in Human normal tonsil.

    References

    ab155991 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
    Permeabilization
    Yes - 0.05% tween 20 in the washing buffer
    Specification
    mouse kidney
    Blocking step
    commercial - Invitrogen's CAS-Block as blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 21 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (adult and fetal kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
    Permeabilization
    Yes - 0.05% tween 20 in the washing buffer
    Specification
    adult and fetal kidney
    Blocking step
    commercial - Invitrogen's CAS-Block as blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 03 2015

    Application
    Flow Cytometry
    Fixation
    live non-fixed cells
    Permeabilization
    No
    Sample
    Human Cell (hESC H9)
    Specification
    hESC H9
    Gating Strategy
    Doublet discrimination gating/live-dead discrimination (PI)/GFP-expressing cells
    Preparation
    Cell harvesting/tissue preparation method: mild trypsinisation/non-enzymatic dissociation
    Sample buffer: FACS staining buffer
    Username

    Dr. Dmitry Shaposhnikov

    Verified customer

    Submitted Nov 05 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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