Anti-Aminopeptidase A antibody [OTI1C4] (ab119798)

Overview

  • Product nameAnti-Aminopeptidase A antibody [OTI1C4]
    See all Aminopeptidase A primary antibodies
  • Description
    Mouse monoclonal [OTI1C4] to Aminopeptidase A
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Aminopeptidase A. Produced in HEK293T cells.
    Database link: NP_001968

  • Positive control
    • HEK293T cells transfected with Aminopeptidase A, Human Kidney, Carcinoma of Human kidney, Human liver and Human endometrium tissues, COS7 cells transiently transfected with Aminopeptidase A, Hela cells
  • General notes

    The clone number has been updated from 1C4 to OTI1C4, both clone numbers name the same clone.

    Dilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab119798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 109 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionAppears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
  • Tissue specificityExpressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
  • Sequence similaritiesBelongs to the peptidase M1 family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aminopeptidase A antibody
    • AMPE_HUMAN antibody
    • AP-A antibody
    • APA antibody
    • bp-1 antibody
    • bp1 antibody
    • CD249 antibody
    • Differentiation antigen gp160 antibody
    • EAP antibody
    • Enpep antibody
    • Glutamyl aminopeptidase (aminopeptidase A) antibody
    • Glutamyl aminopeptidase antibody
    • Gp160 antibody
    • Ly51 antibody
    see all

Anti-Aminopeptidase A antibody [OTI1C4] images

  • All lanes : Anti-Aminopeptidase A antibody [OTI1C4] (ab119798) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY BP1

    Lysates/proteins at 5 µg/ml per lane.


    Predicted band size : 109 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210521)
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue using ab119798 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human Kidney tissue using ab119798 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human liver tissue using ab119798 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human endometrium tissue using ab119798 at a dilution of 1/150.
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BP1 using ab119798 at a dilution of 1/100.
  • HEK293T cells transfected with either RC210521 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119798 at a dilution of 1/100, and then analyzed by flow cytometry.
  • Flow cytometric Analysis of Hela cells, using ab119798 at a dilution of 1/100 (Red), compared to a nonspecific negative control antibody (Blue).

References for Anti-Aminopeptidase A antibody [OTI1C4] (ab119798)

ab119798 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Permeabilization No
Sample Human Cell (hESC H9)
Specification hESC H9
Gating Strategy Doublet discrimination gating/live-dead discrimination (PI)/GFP-expressing cells
Preparation Cell harvesting/tissue preparation method: mild trypsinisation/non-enzymatic dissociation
Sample buffer: FACS staining buffer
Username

Dr. Dmitry Shaposhnikov

Verified customer

Submitted Dec 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"