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Ammonia Assay Kit (ab83360) provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia or Ammonium. In the assay, Ammonia or Ammonium is converted to a product that reacts with the OxiRed probe to generate color (?max = 570 nm) which can be easily quantified by plate reader. The kit can detect 1 nmol (~20 µM) of ammonia or ammonium, much more sensitive than measuring NADPH based ammonia assay.
Visit our FAQs page for tips and troubleshooting.
Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance.
|Ammonia Assay Buffer||WM||1 x 25ml|
|Converting Enzyme (Lyophilized)||Blue||1 vial|
|Enzyme Mix (lyophilized)||Green||1 vial|
|NH4Cl Standard||Yellow||1 x 100µl|
|OxiRed Probe in DMSO||Red||1 x 200µl|
Our Abpromise guarantee covers the use of ab83360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Ammonia measured in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).
Ammonia measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).
Ammonia measured in cell lysates, background signal subtracted (duplicates +/- SD).
Ammonia measured in biological fluids, background signal subtracted (duplicates +/- SD).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"