Overview

  • Product name
    Ammonia Assay Kit
    See all Ammonia kits
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Quantitative
  • Sensitivity
    20 µM
  • Assay time
    1h 00m
  • Product overview

    Ammonia Assay Kit (ab83360) provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of ammonia and ammonium. In the assay, ammonia and ammonium are converted to a product that reacts with the OxiRed probe to generate color (λmax = 570 nm) which can be easily quantified by plate reader. The kit can detect 1 nmol (~20 µM) of total ammonia and ammonium, which is much more sensitive than measuring ammonia with a NADPH based assay.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Ammonia Assay Buffer WM 1 x 25ml
    Converting Enzyme (Lyophilized) Blue 1 vial
    Developer Orange 1 vial
    Enzyme Mix (lyophilized) Green 1 vial
    NH4Cl Standard Yellow 1 x 100µl
    OxiRed Probe in DMSO Red 1 x 200µl
  • Research areas
  • Relevance
    Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.

Applications

Our Abpromise guarantee covers the use of ab83360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Ammonia measured in cell culture medium (range of dilution 1:10-1:200) and control medium (range of dilution 1:1-1:20), background signal subtracted (duplicates +/- SD).

  • Ammonia measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

  • Ammonia measured in cell lysates, background signal subtracted (duplicates +/- SD).

  • Ammonia measured in biological fluids (range of dilution 1:1-1:100), background signal subtracted (duplicates +/- SD).

  • Example of ammonia standard curve using ab83360

Protocols

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting Abcam earlier today.

This kit detects ammonia, but not ammonium. I know that you emailed a week ago, asking a similar question regarding the chemical reaction table for this kit. I am still waiting to hear back from ...

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COMPATIBILITY OF THE KIT WITH ORGANIC SOLVENTS

Excellent Good 4/5 (Ease of Use)
Abreviews
The ammonia determination is accurate for samples of tissue extracted in methanol:acetonitrile:water (5:3:2) solution, provided that the samples are diluted 1:5 in assay buffer. The reaction efficiency decreases when the ratio between organic solvents and assay buffer increases. The reaction does not occur if the samples in organic solvents are not further diluted in assay buffer.
Figure Legend. The standard curve has been generated by mixing in each well 10 microliters of methanol, acetonitrile, water, solution containing known amounts of ammonia, to 40 microliters of assay buffer. The reaction started with the addition of 50microliters/well of reaction mix. Samples were incubated at 37 degrees for 1 hour.
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Abcam user community

Verified customer

Submitted Jul 27 2016

For this kit, we prefer measuring from fresh samples for best results but cell culture media can be centrifuged to remove any debris and then stored at -80C for future assay.

Abreviews
We had good results using the ABCAM ammonia kit for the detection of NH3 in our cell lysates. However, we wanted to see the distribution of NH3 in the intact cells to determine the intercellular variation. We were able to adapt the kit to allow for the in situ detection of ammonia. We chose two cell lines one strongly + for NH3 and one -. The key was to use formalin fixed cells AND to digest in protease prior to use. Under these conditions, we got an excellent signal in the + cells and no signal in the - cells.
Username

Abcam user community

Verified customer

Submitted Dec 27 2013

The kit detects Ammonia which would exist as ammonium ion in solution. The detection principle is based on reaction with ammonium ion in solution. This ammonium ion population includes free pre-existing cellular ammonium and the ionized form of ammonia...

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We have not received any complaints from the other customers who bought this same kit and same lot number. Its possible there was a problem with reconstitution and storage of the kit components.


Important points to consider regarding th...

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For serum or plasma samples, there are no special requirements for preparation of serum or plasma samples for the ammonia assay. For the beta HB assay, it is recommended (see section 4 of the protocol) that samples be de-proteinated with a 10 kDa filte...

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Thank you for contacting us.
In response to your questions on this kit, the pH of the sample should be ˜7-8. To use mouse stool sample, add assay buffer to sample. Mix it completely either by pipetting up & down, sonicating or vortexing. Centr...

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Thanks for your enquiry and for providing the standard curve data.
The standard curve data you provided look good and are quite similar to the example standard curve seen on the online product datasheet for this product. If you could, please furth...

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Thank you for your patience.

I have checked with the lab about more details about the kit's mechanism. Thefollowingdetails are the ones they can give out:

Our assay buffer contains some ingredients which can interact with the ammon...

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1-10 of 25 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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