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Abcam's Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (ab102509) provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In this non-enzymatic assay, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (λ=670nm) using a plate reader.
Visit our FAQs page for tips and troubleshooting.
The kit can detect less than 1 nmol(~10 µM) ammonia or ammonium using either a 96 well or 384 well microwell plate.
Store the kit at +4°C.
|Ammonia Reagent 1||Amber||1 x 8ml|
|Ammonia Reagent 2||Clear||1 x 4ml|
|Ammonium Chloride Standard||Yellow||1 x 0.1ml|
Our Abpromise guarantee covers the use of ab102509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Standard curve: mean of duplicates (+/- SD) with background reads subtracted.
Ammonia measured in biological fluids showing quantity (mM) in tested samples. Samples were diluted 10-1000 fold.
ab102509 has not yet been referenced specifically in any publications.