Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (ab102509)
Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture media, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 10 µM
Overview
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Product name
Ammonia Assay Kit - Modified Berthelot - (Colorimetric)
See all Ammonia kits -
Sample type
Urine, Serum, Plasma, Other biological fluids, Cell culture media -
Assay type
Quantitative -
Sensitivity
< 10 µM -
Assay time
0h 30m -
Product overview
Ammonia Assay Kit - Modified Berthelot (Colorimetric) ab102509 provides a rapid, simple, sensitive, and reliable assay to measure ammonia.
In this non-enzymatic ammonia assay protocol, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (λ=670nm) using a plate reader.
The kit can detect less than 1 nmol(~10 µM) ammonia or ammonium using either a 96 well or 384 well microwell plate.
Ammonia assay protocol summary:
- add samples and standards to wells
- add reagent 1 and reagent 2 and incubate for 30 min
- analyze with a microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K470 Ammonia Colorimetric Assay Kit II. K470-100 is the same size as the 100 test size of ab102509.
Ammonia assay kit ab83360 is an alternative to this product, and is the most popular ammonia assay kit.
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Reagent II 1 x 8ml Reagent III 1 x 4ml Ammonium Standard II 1 x 0.1ml -
Research areas
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Relevance
Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab102509 has been referenced in 5 publications.
- Srinivasachari S et al. A dimer between monomers and hexamers-Oligomeric variations in glucosamine-6-phosphate deaminase family. PLoS One 18:e0271654 (2023). PubMed: 36598911
- Fleming D et al. Contribution of Uremia to Ureaplasma-Induced Hyperammonemia. Microbiol Spectr 10:e0194221 (2022). PubMed: 35171026
- Pettinato G et al. Spectroscopic label-free microscopy of changes in live cell chromatin and biochemical composition in transplantable organoids. Sci Adv 7:N/A (2021). PubMed: 34407934
- Raghavan SS et al. Development and application of a transcriptional sensor for detection of heterologous acrylic acid production in E. coli. Microb Cell Fact 18:139 (2019). PubMed: 31426802
- Wu C et al. Fluxomic Analysis Reveals Central Carbon Metabolism Adaptation for Diazotroph Azotobacter vinelandii Ammonium Excretion. Sci Rep 9:13209 (2019). PubMed: 31520074