Overview

  • Product nameAnti-AMPD1 antibody
    See all AMPD1 primary antibodies
  • Description
    Rabbit polyclonal to AMPD1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, Pig, Monkey
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region between residues 140 and 190 of human AMPD1 (NP_000027).

  • Positive control
    • Mouse and rat skeletal muscle lysates. Human skeletal muscle tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • RelevanceAMP deaminase (AMPD) is an allosteric enzyme that plays a critical role in energy metabolism. There are three functional isoforms of AMPD. AMPD1 is the skeletal muscle-specific isoform M located in type II muscle fibers, neuromuscular junctions and in capillaries. Defects in AMPD1 are the cause of adenosine monophosphate deaminase deficiency muscle type (AMPDDM). AMPDDM is a metabolic disorder resulting in exercise-related myopathy. It is characterized by exercise-induced muscle aches, cramps, and early fatigue.
  • Database links
  • Alternative names
    • Adenosine monophosphate deaminase 1 (isoform M) antibody
    • Adenosine monophosphate deaminase 1 antibody
    • AMP deaminase 1 antibody
    • AMP deaminase isoform M antibody
    • AMPD 1 antibody
    • AMPD antibody
    • Ampd01 antibody
    • MAD antibody
    • MADA antibody
    • Myoadenylate deaminase antibody
    • RATAMPD01 antibody
    • Skeletal muscle AMPD antibody
    see all

Anti-AMPD1 antibody images

  • All lanes : Anti-AMPD1 antibody (ab72541) at 1 µg/ml

    Lane 1 : Mouse skeletal muscle lysate
    Lane 2 : Mouse skeletal muscle lysate with immunising peptide
    Lane 3 : Rat skeletal muscle lysate


    Predicted band size : 87 kDa
    Observed band size : 87 kDa
    Additional bands at : 10 kDa. We are unsure as to the identity of these extra bands.
  • ab72541 at 10µg/ml staining AMPD1 in human skeletal muscle by Immunohistochemistry using paraffin-embedded tissue.

References for Anti-AMPD1 antibody (ab72541)

ab72541 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Spermophilus tridecemlineatus Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Mr. James Bjork

Verified customer

Submitted Aug 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% 37.5:1)
Sample Pig Tissue lysate - whole (Skeletal Muscle)
Specification Skeletal Muscle
Blocking step Thermo Starting Block 37542 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Eric England

Verified customer

Submitted Jun 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"