The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 92 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionAMP deaminase plays a critical role in energy metabolism.
Tissue specificityThree isoforms are present in mammals: AMP deaminase 1 is the predominant form in skeletal muscle; AMP deaminase 2 predominates in smooth muscle, non-muscle tissue, embryonic muscle and undifferentiated myoblasts; AMP deaminase 3 is found in erythrocytes.
PathwayPurine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.
Sequence similaritiesBelongs to the adenosine and AMP deaminases family.