Overview

  • Product nameAnti-Amphiphysin antibody
    See all Amphiphysin primary antibodies
  • Description
    Goat polyclonal to Amphiphysin
  • SpecificityThis antibody is expected to recognize both reported isoforms (NP_001626.1 and NP_647477.1) of Amphiphysin.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Dog, Human
  • Immunogen

    Synthetic peptide:

    C-KRKDESRISKAE

    , corresponding to internal sequence amino acids 159-170 of Human Amphiphysin

  • Positive control
    • Mouse Brain lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Antibody detection limit dilution 1/16,000.

    WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 76 kDa). The observed molecular weight corresponds to earlier findings in literature (Wigge et al, Mol Biol Cell. 1997 Oct;8(10):2003-15.; PMID: 9348539). A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
    • Tissue specificityNeurons, certain endocrine cell types and spermatocytes.
    • Sequence similaritiesContains 1 BAR domain.
      Contains 1 SH3 domain.
    • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Amp antibody
      • AMPH 1 antibody
      • AMPH antibody
      • AMPH_HUMAN antibody
      • AMPH1 antibody
      • Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen) antibody
      • Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) antibody
      • Amphiphysin antibody
      • Amphiphysin I antibody
      • CG8604 antibody
      • Damp antibody
      • dAmph antibody
      • Dmel\CG8604 antibody
      • HGNC:471 antibody
      • MGC49429 antibody
      • Stiff man syndrome with breast cancer 128kD autoantigen antibody
      • Stiff man syndrome with breast cancer 128kDa autoantigen antibody
      see all

    Anti-Amphiphysin antibody images

    References for Anti-Amphiphysin antibody (ab50340)

    ab50340 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab50340.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"