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Goat polyclonal to Amphiphysin
This antibody is expected to recognize both reported isoforms (NP_001626.1 and NP_647477.1) of Amphiphysin.
Predicted to work with:
Rat, Dog, Human
, corresponding to internal sequence amino acids 159-170 of Human Amphiphysin
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Antibody detection limit dilution 1/16,000.
WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 76 kDa). The observed molecular weight corresponds to earlier findings in literature (Wigge et al, Mol Biol Cell. 1997 Oct;8(10):2003-15.; PMID: 9348539). A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Neurons, certain endocrine cell types and spermatocytes.
Contains 1 BAR domain.
Contains 1 SH3 domain.
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
Information by UniProt
AMPH 1 antibody
Western blot - Anti-Amphiphysin antibody (ab50340)
Anti-Amphiphysin antibody (ab50340) at 0.1 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer).
Predicted band size:
Observed band size:
125 kDa (
why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"