Overview

  • Product name
  • Description
    Goat polyclonal to Amphiphysin
  • Specificity
    This antibody is expected to recognize both reported isoforms (NP_001626.1 and NP_647477.1) of Amphiphysin.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Dog, Human
  • Immunogen

    Synthetic peptide:

    C-KRKDESRISKAE

    , corresponding to internal sequence amino acids 159-170 of Human Amphiphysin

  • Positive control
    • Mouse Brain lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/16,000.

    WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 76 kDa). The observed molecular weight corresponds to earlier findings in literature (Wigge et al, Mol Biol Cell. 1997 Oct;8(10):2003-15.; PMID: 9348539). A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
    • Tissue specificity
      Neurons, certain endocrine cell types and spermatocytes.
    • Sequence similarities
      Contains 1 BAR domain.
      Contains 1 SH3 domain.
    • Cellular localization
      Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Amp antibody
      • AMPH 1 antibody
      • AMPH antibody
      • AMPH_HUMAN antibody
      • AMPH1 antibody
      • Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen) antibody
      • Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) antibody
      • Amphiphysin antibody
      • Amphiphysin I antibody
      • CG8604 antibody
      • Damp antibody
      • dAmph antibody
      • Dmel\CG8604 antibody
      • HGNC:471 antibody
      • MGC49429 antibody
      • Stiff man syndrome with breast cancer 128kD autoantigen antibody
      • Stiff man syndrome with breast cancer 128kDa autoantigen antibody
      see all

    Images

    References

    ab50340 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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