Anti-Amphiphysin antibody [C14-23] (ab16770)

Overview

  • Product nameAnti-Amphiphysin antibody [C14-23]
    See all Amphiphysin primary antibodies
  • Description
    Mouse monoclonal [C14-23] to Amphiphysin
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, ELISA, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fusion protein, corresponding to amino acids 320-696 of Human Amphiphysin.

  • Positive control
    • Mouse brain extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.03% Sodium Azide
    Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberC14-23
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 76 kDa.
IP Use a concentration of 1 - 2 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionMay participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
  • Tissue specificityNeurons, certain endocrine cell types and spermatocytes.
  • Sequence similaritiesContains 1 BAR domain.
    Contains 1 SH3 domain.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amp antibody
    • AMPH 1 antibody
    • AMPH antibody
    • AMPH_HUMAN antibody
    • AMPH1 antibody
    • Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen) antibody
    • Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) antibody
    • Amphiphysin antibody
    • Amphiphysin I antibody
    • CG8604 antibody
    • Damp antibody
    • dAmph antibody
    • Dmel\CG8604 antibody
    • HGNC:471 antibody
    • MGC49429 antibody
    • Stiff man syndrome with breast cancer 128kD autoantigen antibody
    • Stiff man syndrome with breast cancer 128kDa autoantigen antibody
    see all

Anti-Amphiphysin antibody [C14-23] images



  • Predicted band size : 76 kDa


    ab16770 at 1/2000 dilution staining Amphiphysin in mouse and rat brain by Western blot. Lane 1: Mouse brain. Lane 2: Rat brain. Top band: Amphiphysin 1 Lower band: Amphiphysin 2
  • ICC/IF image of ab16770 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16770, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing SH-SY5Y cells stained with ab16770 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16770, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Amphiphysin antibody [C14-23] (ab16770)

ab16770 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"