Recombinant
RabMAb

Anti-Amphiphysin antibody [EP2060Y] (ab52646)

Overview

  • Product name
    Anti-Amphiphysin antibody [EP2060Y]
    See all Amphiphysin primary antibodies
  • Description
    Rabbit monoclonal [EP2060Y] to Amphiphysin
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues within human Amphipysin.

  • Positive control
    • Human brain lysate, human brain tissue or SH-SYS5 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab52646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/25000 - 1/50000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa).
Flow Cyt 1/15.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
    • Tissue specificity
      Neurons, certain endocrine cell types and spermatocytes.
    • Sequence similarities
      Contains 1 BAR domain.
      Contains 1 SH3 domain.
    • Cellular localization
      Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Amp antibody
      • AMPH 1 antibody
      • AMPH antibody
      • AMPH_HUMAN antibody
      • AMPH1 antibody
      • Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen) antibody
      • Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) antibody
      • Amphiphysin antibody
      • Amphiphysin I antibody
      • CG8604 antibody
      • Damp antibody
      • dAmph antibody
      • Dmel\CG8604 antibody
      • HGNC:471 antibody
      • MGC49429 antibody
      • Stiff man syndrome with breast cancer 128kD autoantigen antibody
      • Stiff man syndrome with breast cancer 128kDa autoantigen antibody
      see all

    Images

    • Anti-Amphiphysin antibody [EP2060Y] (ab52646) at 1/100000 dilution + Human brain lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 76 kDa
      Observed band size : 128 kDa (why is the actual band size different from the predicted?)
    • Ab52646 (1/100) staining human Amphiphysin in human brain tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunofluorescent staining of SH-SYS5 cells using ab52646 (1/100).
    • Overlay histogram showing SH-SY5Y cells stained with ab52646 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52646, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References

    This product has been referenced in:
    • Bosch PJ  et al. Proteomics Analysis of Dorsal Striatum Reveals Changes in Synaptosomal Proteins following Methamphetamine Self-Administration in Rats. PLoS One 10:e0139829 (2015). WB . Read more (PubMed: 26484527) »
    • Kumar GS  et al. Quantitative proteomics for identifying biomarkers for tuberculous meningitis. Clin Proteomics 9:12 (2012). IHC . Read more (PubMed: 23198679) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab52646.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up