Anti-Amphiphysin antibody [EP2061Y] (ab76124)

Overview

  • Product nameAnti-Amphiphysin antibody [EP2061Y]
    See all Amphiphysin primary antibodies
  • Description
    Rabbit monoclonal [EP2061Y] to Amphiphysin
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human Amphiphysin

  • Positive control
    • Human, mouse and rat brain tissue lysates; human brain tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab76124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000 - 1/100000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa).
IP 1/20.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
  • Tissue specificityNeurons, certain endocrine cell types and spermatocytes.
  • Sequence similaritiesContains 1 BAR domain.
    Contains 1 SH3 domain.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amp antibody
    • AMPH 1 antibody
    • AMPH antibody
    • AMPH_HUMAN antibody
    • AMPH1 antibody
    • Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen) antibody
    • Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) antibody
    • Amphiphysin antibody
    • Amphiphysin I antibody
    • CG8604 antibody
    • Damp antibody
    • dAmph antibody
    • Dmel\CG8604 antibody
    • HGNC:471 antibody
    • MGC49429 antibody
    • Stiff man syndrome with breast cancer 128kD autoantigen antibody
    • Stiff man syndrome with breast cancer 128kDa autoantigen antibody
    see all

Anti-Amphiphysin antibody [EP2061Y] images

  • ab76124 at 1/100 dilution, staining Amphiphysin in human brain by Immunohistochemistry using paraffin-embedded tissue.
  • All lanes : Anti-Amphiphysin antibody [EP2061Y] (ab76124) at 1/100000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 76 kDa
    Observed band size : 128 kDa (why is the actual band size different from the predicted?)

References for Anti-Amphiphysin antibody [EP2061Y] (ab76124)

ab76124 has not yet been referenced specifically in any publications.

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