Anti-Amphiregulin antibody (ab180722)

Overview

  • Product name
    Anti-Amphiregulin antibody
    See all Amphiregulin primary antibodies
  • Description
    Rabbit polyclonal to Amphiregulin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Human Amphiregulin aa 20-252.
    Database link: P15514

  • Positive control
    • MCF7, HT29 and A549 cell extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 28 kDa.

Target

  • Function
    Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
  • Sequence similarities
    Belongs to the amphiregulin family.
    Contains 1 EGF-like domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A REG antibody
    • Amphiregulin antibody
    • Amphiregulin B antibody
    • AR antibody
    • AREG antibody
    • AREG_HUMAN antibody
    • AREGB antibody
    • Colorectum cell derived growth factor antibody
    • Colorectum cell-derived growth factor antibody
    • CRDGF antibody
    • MGC13647 antibody
    • Schwannoma derived growth factor antibody
    • SDGF antibody
    see all

Images

  • All lanes : Anti-Amphiregulin antibody (ab180722) at 1/500 dilution

    Lane 1 : MCF7 cell extracts
    Lane 2 : A549 cell extracts
    Lane 3 : HT29 cell extracts


    Predicted band size : 28 kDa

References

ab180722 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 25% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 08 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (MCF7 cells)
Specification
MCF7 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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