Anti-Amphiregulin antibody - BSA and Azide free (ab31344)

Overview

  • Product nameAnti-Amphiregulin antibody - BSA and Azide free
    See all Amphiregulin primary antibodies
  • Description
    Rabbit polyclonal to Amphiregulin - BSA and Azide free
  • SpecificityHighly specific to amphiregulin. No cross-reactivity with EGF, TGFa, or HB-EGF.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    RKKKGGKNGKNRRNRKKKN

    , corresponding to amino acids 26-44 of secreted Human Amphiregulin

  • Positive control
    • LS174T; phorbol ester treated MCF-7 breast cancer cells; epithelial cells of mucosal surface in normal colon.
  • General notes


    Directed against the region of amphiregulin which binds to heparin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesDirected against the region of amphiregulin which binds to heparin.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 28 kDa.
IP Use at an assay dependent dilution.

Target

  • FunctionBifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
  • Sequence similaritiesBelongs to the amphiregulin family.
    Contains 1 EGF-like domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A REG antibody
    • Amphiregulin antibody
    • Amphiregulin B antibody
    • AR antibody
    • AREG antibody
    • AREG_HUMAN antibody
    • AREGB antibody
    • Colorectum cell derived growth factor antibody
    • Colorectum cell-derived growth factor antibody
    • CRDGF antibody
    • MGC13647 antibody
    • Schwannoma derived growth factor antibody
    • SDGF antibody
    see all

References for Anti-Amphiregulin antibody - BSA and Azide free (ab31344)

ab31344 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mammary gland)
Specification Mammary gland
Fixative Acetone:methanol (1:1)
Permeabilization Yes - 0.025% Triton X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Dec 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"