AMPK alpha 1 293T Transfected Lysate - (positive control) (ab94311)

Overview

  • Product nameAMPK alpha 1 293T Transfected Lysate - (positive control)
  • General notesab94311 is a 293T cell transfected lysate in which Human AMPK alpha 1 has been transiently over-expressed using a pCMV-AMPK alpha 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.

Applications

Our Abpromise guarantee covers the use of ab94311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • AMPK alpha 1 293T Transfected Lysate - (positive control) images

    • ab94311 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-AMPK alpha 1 antibody (ab89214) at 1/500 dilution

      Lane 1 : AMPK alpha 1 293T Transfected Lysate - Positive Control (ab94311)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

    References for AMPK alpha 1 293T Transfected Lysate - (positive control) (ab94311)

    ab94311 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"