Anti-AMPK alpha 1 + AMPK alpha 2 antibody (ab39644)

Overview

  • Product nameAnti-AMPK alpha 1 + AMPK alpha 2 antibody
    See all AMPK alpha 1 + AMPK alpha 2 primary antibodies
  • Description
    Rabbit polyclonal to AMPK alpha 1 + AMPK alpha 2
  • Specificityab39644 detects endogenous levels of total AMPK alpha 1/AMPK alpha 2 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (non-phosphorylated) derived from human AMPK alpha 1/AMPK alpha 2 around the phosphorylation site of Serine 485 or Serine 491.

  • Positive control
    • Human colon carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab39644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 62 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • FunctionCatalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • DomainThe AIS (autoinhibitory sequence) region some sequence similarity with the ubiquitin-associated domains and represses kinase activity.
  • Post-translational
    modifications
    Ubiquitinated.
    Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at a much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
  • Cellular localizationCytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • Acetyl-CoA carboxylase kinase antibody
    • AMPK alpha 1 chain antibody
    • AMPK alpha 2 chain antibody
    • AMPK antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPK2 antibody
    • HMGCR kinase antibody
    • Hydroxymethylglutaryl-CoA reductase kinase antibody
    • PRKAA antibody
    • PRKAA1 antibody
    • PRKAA2 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • Protein kinase AMP activated alpha 2 catalytic subunit antibody
    • Tau-protein kinase PRKAA1 antibody
    see all

Anti-AMPK alpha 1 + AMPK alpha 2 antibody images

  • ab39644, at 1/50 dilution, staining AMPK alpha 1 + AMPK alpha 2 in Human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue.

References for Anti-AMPK alpha 1 + AMPK alpha 2 antibody (ab39644)

This product has been referenced in:
  • Stone JD  et al. AMP-activated protein kinase inhibits vascular smooth muscle cell proliferation and migration and vascular remodeling following injury. Am J Physiol Heart Circ Physiol 304:H369-81 (2013). Rat . Read more (PubMed: 23203966) »
  • Mendoza EE  et al. Control of Glycolytic Flux by AMP-Activated Protein Kinase in Tumor Cells Adapted to Low pH. Transl Oncol 5:208-16 (2012). WB ; Human, Rat . Read more (PubMed: 22741040) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Horse Cell lysate - whole cell (Jejunum epithelium)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification Jejunum epithelium
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 01 2016

Thank you for contacting us and for providing the horse sequence. I have checked the homology between human and horse for both immunogens, and the results are as follows: AMPK alpha 1: homology around Serine 485 = very poor (54%) AMPK alpha 2: ...

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