Overview

  • Product nameAnti-AMPK alpha 1 antibody
    See all AMPK alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to AMPK alpha 1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of AMP activated protein Kinase (AMPK), alpha 1 catalytic subunit encoded within exon 8.

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas It also gave a positive result in PANC1 cell line in IF/ICC

Applications

Our Abpromise guarantee covers the use of ab3759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
IP Use at 2-8 µg/mg of lysate.
WB 1/500 - 1/5000. Predicted molecular weight: 63 kDa.

Target

  • FunctionResponsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC 2.7.11.1 antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all

Anti-AMPK alpha 1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling AMPK alpha 1 with ab3759 at 1/5000 (0.2µg/ml). Detection: DAB.


  • Predicted band size : 63 kDa

    Samples: Extracts from

    1. Bovine aortic endothelial cells

    2. Rat aortic smooth muscle cells

    3. HepG2 cells

    4. Human aortic endothelial cells

    Antibody: Affinity purified Rabbit anti-AMPK alpha 1 (ab3759) used at 2ug/ml (1/500).

     

    Samples: Extracts from

    1. Bovine aortic endothelial cells

    2. Rat aortic smooth muscle cells

    3. HepG2 cells

    4. Human aortic endothelial cells

    Antibody: Affinity purified Rabbit anti-AMPK alpha 1 (ab3759) used at 2ug/ml (1/500).

  • IHC image of AMPK alpha 1 staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3759, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab3759 stained PANC1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3759, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-AMPK alpha 1 antibody (ab3759)

This product has been referenced in:
  • Mitterberger MC & Zwerschke W Mechanisms of Resveratrol-Induced Inhibition of Clonal Expansion and Terminal Adipogenic Differentiation in 3T3-L1 Preadipocytes. J Gerontol A Biol Sci Med Sci N/A:N/A (2013). Read more (PubMed: 23525482) »
  • Lieberthal W  et al. Susceptibility to ATP depletion of primary proximal tubular cell cultures derived from mice lacking either the a1 or the a2 isoform of the catalytic domain of AMPK. BMC Nephrol 14:251 (2013). Mouse . Read more (PubMed: 24228806) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us.

Unfortunately, we have not tested this antibody for cross reactivity with AMPK alpha 2 and are unsure whether it will cross react with this protein as we do not have any data for support.

We do offer...

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Thank you for your enquiry. Our source for ab3759 and ab3760 has told me that the antibodies do not cross-react with the homologs. If you have any additional questions, please contact us again.

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in re...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"