The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa.
FunctionResponsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.
5 AMP activated protein kinase alpha 1catalytic subunit antibody
5 AMP activated protein kinase catalytic alpha 1 chain antibody
5' AMP activated protein kinase catalytic subunit alpha 1 antibody
5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
ACACA kinase antibody
acetyl CoA carboxylase kinase antibody
AMP -activate kinase alpha 1 subunit antibody
AMP-activated protein kinase, catalytic, alpha -1 antibody
AMPK 1 antibody
AMPK alpha 1 antibody
AMPK alpha 1 chain antibody
AMPK subunit alpha-1 antibody
EC 18.104.22.168 antibody
HMG CoA reductase kinase antibody
HMGCR kinase antibody
hormone sensitive lipase kinase antibody
Hydroxymethylglutaryl CoA reductase kinase antibody
kinase AMPK alpha1 antibody
PRKAA 1 antibody
Protein kinase AMP activated alpha 1 catalytic subunit antibody
SNF1-like protein AMPK antibody
Tau protein kinase PRKAA1 antibody
Anti-AMPK alpha 1 antibody - Aminoterminal end images
Western blot - AMPK alpha 1 antibody (ab49656)
Lanes 1 - 3 : Anti-AMPK alpha 1 antibody - Aminoterminal end (ab49656) at 1/4000 dilution Lane 4 : Anti-AMPK alpha 1 antibody - Aminoterminal end (ab49656) at 1/8000 dilution Lane 5 : Anti-AMPK alpha 1 antibody - Aminoterminal end (ab49656) at 1/10000 dilution
Lane 1 : undifferentiated embryonic 3T3-L1 cells Lane 2 : 3T3-L1 differentiated into adipocytes by treatment with dexamethasone and insulin.
with Inhibition with AMPKa (human 2-19) immunizing peptide.
at 20 µg/ml Lane 3 : 3T3-L1 differentiated into adipocytes by treatment with dexamethasone and insulin.
Lane 4 : 3T3-L1 differentiated into adipocytes by treatment with dexamethasone and insulin.
Lane 5 : 3T3-L1 differentiated into adipocytes by treatment with dexamethasone and insulin.
Secondary HRP conjugated Goat Anti-Rabbit IgG
Predicted band size : 60 kDa Observed band size : 60 kDa
References for Anti-AMPK alpha 1 antibody - Aminoterminal end (ab49656)
has not yet been referenced specifically in any publications.
Publishing research using ab49656? Please let us know so that we can cite the reference in this datasheet.
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