Anti-AMPK alpha 1 antibody - Aminoterminal end (ab49656)

Overview

  • Product nameAnti-AMPK alpha 1 antibody - Aminoterminal end
    See all AMPK alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to AMPK alpha 1 - Aminoterminal end
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    ATAEKQKHDGRVKIGHYI

    conjugated to KLH, corresponding to N terminal amino acids 2-19 of Human AMPK alpha 1

  • Positive control
    • Whole cell extract of mouse 3T3-L1 adipocytes and a whole cell extract of human kidney 293 cell line.

Applications

Our Abpromise guarantee covers the use of ab49656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa.

Target

  • FunctionResponsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC 2.7.11.1 antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all

Anti-AMPK alpha 1 antibody - Aminoterminal end images

  • Lanes 1 - 3 : Anti-AMPK alpha 1 antibody - Aminoterminal end (ab49656) at 1/4000 dilution
    Lane 4 : Anti-AMPK alpha 1 antibody - Aminoterminal end (ab49656) at 1/8000 dilution
    Lane 5 : Anti-AMPK alpha 1 antibody - Aminoterminal end (ab49656) at 1/10000 dilution

    Lane 1 : undifferentiated embryonic 3T3-L1 cells
    Lane 2 : 3T3-L1 differentiated into adipocytes by treatment with dexamethasone and insulin. with Inhibition with AMPKa (human 2-19) immunizing peptide. at 20 µg/ml
    Lane 3 : 3T3-L1 differentiated into adipocytes by treatment with dexamethasone and insulin.
    Lane 4 : 3T3-L1 differentiated into adipocytes by treatment with dexamethasone and insulin.
    Lane 5 : 3T3-L1 differentiated into adipocytes by treatment with dexamethasone and insulin.

    Secondary
    HRP conjugated Goat Anti-Rabbit IgG

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

References for Anti-AMPK alpha 1 antibody - Aminoterminal end (ab49656)

ab49656 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"